摘要
【目的】揭示完全不育花与可育花内源激素含量的差异,筛选参与完全不育花不育表型形成的关键内源激素,为研究完全不育花败育机制提供参考依据。【方法】通过高效液相色谱—电喷雾串联质谱法对可育茶树佛香2号(母本)和福鼎大白茶(父本),以及完全不育茶树云茶异蕊(杂交子一代)的花蕾和盛开花进行22种内源激素及相关代谢物的检测,并对内源激素及相关代谢物含量进行相关分析,以及不同品种间的主成分分析和聚类分析。【结果】与可育花相比,云茶异蕊不同花期均具有较高含量的L-色氨酸(Trp)、色胺(TAM)、吲哚-3-乙酸(IAA)、IAA-天冬氨酸(IAA-Asp)和玉米素(ZT),而吲哚-3-乙酰胺(IAM)、异戊烯基腺嘌呤(IP)和异戊烯基腺苷(IPA)的含量较低;反式玉米素(TZ)、反式玉米素核苷(TZR)和二氢玉米素核苷(DZR)整体的变化趋势一致,其在父母本中均为花蕾的含量极显著高于盛开花(P<0.01,下同),在云茶中则相反;云茶中赤霉素A_(4)(GA_(4))和赤霉素A_(7)(GA_(7))含量始终极显著高于父母本,而脱落酸(ABA)含量极显著低于父母本;茉莉酸(JA)在云茶盛开花中的含量极显著高于花蕾,而父母本与之相反,茉莉酸—异亮氨酸(JA-Ile)不论是花蕾还是盛开花含量均极显著低于同时期的父母本。相关分析结果表明,不同内源激素之间在含量上存在较为复杂的正相关或负相关关系。云茶异蕊与父母本花中内源激素的分布模式存在明显差异,且云茶异蕊花蕾和盛开花聚在一起,具有更相似的内源激素水平。【结论】多种内源激素的盈亏及其相互间的作用共同参与云茶异蕊完全不育表型的形成,其中云茶异蕊较高含量的IAA-Asp、ZT、GA_(4)和GA_(7),以及较低含量的ABA、IP和IPA等可能与其完全不育表型密切相关。
【Objective】To reveal the differences in endogenous hormone contents between completely sterile flowers and fertile flowers,and to screen the key endogenous hormones involved in the formation of sterile phenotypes in com‐pletely sterile flowers,which could provide an important reference for the study of the mechanism of failure of com‐pletely sterile flowers.【Method】The fertile tea plant Foxiang 2(female parent)and Fudingdabaicha(male parent),as well as the completely sterile tea plant Yunchayirui(F1 hybrid),were analyzed for 22 endogenous hormones and related metabolites in both flower buds and fully bloomed flowers using high performance liquid chromatography-electrospray ionization tandem mass spectrometry.Correlation analysis of endogenous hormones and related metabolites was per‐formed,along with principal component analysis and cluster analysis among different varieties.【Result】Compared to fer‐tile flowers,Yunchayirui had higher contents of L-tryptophan(Trp),tryptamine(TAM),indole-3-acetic acid(IAA),IAA-aspartic acid(IAA-Asp)and zeatin(ZT),and lower contents of indole-3-acetamide(IAM),isopentenyladenine(IP)and isopentenyl adenosine(IPA)at different flowering stages.The overall trends of trans-zeatin(TZ),trans-zeatin riboside(TZR)and dihydrozeatin riboside(DZR)were consistent,with their contents being significantly higher in flower buds than in fully bloomed flowers in both parents(P<0.01,the same below),while it was the opposite was ob‐served in Yuncha.The contents of gibberellins A_(4)(GA_(4))and gibberellins A_(7)(GA_(7))in Yuncha were consistently extremely significantly higher than in parents,while abscisic acid(ABA)content was extremely significantly lower than in parental lines.Jasmonic acid(JA)content was extremely significantly higher in Yuncha fully bloomed flowers than in buds,in contrast to the parents,and jasmonoyl-isoleucine(JA-Ile)content was extremely significant lower than the parents at the same time,both in buds and in fully bloomed flowers.The results of the correlation analysis showed that there were more complex positive or negative correlations between different endogenous hormones in terms of content.The distribution pat‐terns of endogenous hormones in Yunchayirui flowers differed markedly from those of the parents,and the flower buds and fully bloomed flowers of Yunchayirui clustered together,indicating more similar endogenous hormone levels.【Con‐clusion】The surpluses and deficits of multiple endogenous hormones,as well as their interactions,are jointly involved in the formation of the completely sterile phenotype of Yunchayirui.Moreover,the higher contents of IAA-Asp,ZT,GA_(4) and GA_(7),and the lower contents of ABA,IP and IPA in Yunchayirui may be closely related to its completely sterile phe‐notype.
作者
庞丹丹
刘玉飞
蒋会兵
田易萍
陈春林
许燕
邓少春
陈林波
PANG Dan-dan;LIU Yu-fei;JIANG Hui-bing;TIAN Yi-ping;CHEN Chun-lin;XU Yan;DENG Shao-chun;CHEN Lin-bo(Tea Research Institute,Yunnan Academy of Agricultural Sciences/Yunnan Key Laboratory for Tea Science/Yunnan Technology Engineering Research Center of Tea Germplasm Innovation and Supporting Cultivation,Xishuangbanna,Yunnan 666201,China)
出处
《南方农业学报》
北大核心
2025年第4期1235-1244,共10页
Journal of Southern Agriculture
基金
国家自然科学基金项目(32060699,32260784)
云南省基础研究计划项目(202201AT070060)
云南省农业基础研究联合专项(202101BD070001-035)。
关键词
茶树
花发育
雌雄不育(完全不育)
内源激素
tea plant
flower development
male and female sterility(completely sterile)
endogenous hormones
