摘要
[目的]旨在开发一种简便的可光固化的胶原基生物墨水。[方法]检测不同浓度胶原蛋白(1~8 mg/mL)溶液的流变特性,考察不同浓度胶原蛋白的可打印性;考察不同浓度核黄素(0~30μg/mL)与胶原蛋白溶液紫外光照射后的交联程度,优化核黄素浓度;使用CCK8和Calcein/PI染色法,检测不同浓度核黄素对L929的细胞毒性;使用微量注射泵对生物墨水进行打印并在365 nm紫外照射下交联。[结果]4~8 mg/mL胶原蛋白溶液的黏度适宜挤出打印;当核黄素浓度达到30μg/mL时,交联度较高为72.51%;CCK8与Calcein/PI染色结果表明,0~100μg/mL核黄素作用于L929细胞24 h后,与对照组相比细胞增殖和细胞存活率均无明显差异;制备的胶原蛋白/核黄素生物墨水经打印和光交联后可以维持其打印形状。[结论]成功制备流变性能良好的胶原蛋白/核黄素生物墨水,且无明显细胞毒性,在356 nm紫外照射下交联固化,证实其可用于生物打印。
[Objective]To develop a simple and light-curable collagen-based bioink.[Method]The viscosity of different concentrations of collagen(1-8 mg/mL)solution was measured to determine the appropriate concentration of collagen;the cross-linking degree of riboflavin(0-30μg/mL)and collagen solution was investigated after UV irradiation,and the appropriate riboflavin concentration was optimized.The cytotoxicity of riboflavin of different concentrations was analyzed by CCK8 and Calcein/PI staining.The microinjection pump was applied to print the bioink and was crosslinked under 365 nm UV irradiation.[Result]The collagen solution of 4-8 mg/mL is suitable for extrusion printing;when the concentration of riboflavin reaches 30μg/mL,the cross-linking degree is 72.51%.The results of CCK8 and Calcein/PI staining showed that there was no significant change in cell proliferation and cell survival rate compared with the control group when L929 cells were treated with 0-100μg/mL riboflavin for 24 hours.The printed shape of the prepared collagen/riboflavin bioink can be maintained after extrusion printing and photocrosslinking.[Conclusion]Collagen/riboflavin bioink with proper rheological properties was prepared,and it exhibited good biocompatibility,photo-curability under 356 nm ultraviolet,and printability.
作者
杨光
陈帅
杨育灿
田炎坤
黄毅
彭新生
陈慧芝
周郁斌
YANG Guang;CHEN Shuai;YANG Yucan;TIAN Yankun;HUANG Yi;PENG Xinsheng;CHEN Huizhi;ZHOU Yubin(Guangdong Provincial Key Laboratory of Research and Development of Natural Drugs,and School of Pharmacy,Guangdong Medical University)
出处
《生物技术》
2025年第2期177-181,198,共6页
Biotechnology
基金
广东省基础与应用基础研究基金项目(2021B1515140006)
广东省普通高校特色创新项目(2022KTSCX042)
国家级和省级大学生创新创业训练计划项目(202210571016,S202210571050,S202210571119,202310571001,202310571004,202310571024)
广东医科大学博士学位人员科研启动基金(GDMUB2021022)。
