摘要
目的 观察中药参佛胃康对胃癌前病变大鼠糖原合酶激酶-3β(GSK-3β)、抑癌基因PTEN及三磷酸磷脂酰肌醇(PIP3)蛋白表达的影响,探讨参佛胃康逆转胃癌前病变的可能机制。方法 将50只Wistar大鼠随机分为10只空白组,其余只通过MNNG综合造模法建立胃癌前病变(PLGC)大鼠模型,造模成功大鼠分为模型组、参佛胃康组、丽珠得乐组。各组大鼠按相应药物进行处理,持续4 w,观察大鼠体重、食量及精神状态,苏木精-伊红(HE)染色观察胃黏膜病理变化,Western Blot法观测胃组织PTEN、GSK-3β及PIP3蛋白表达情况。结果 参佛胃康可有效改善PLGC大鼠一般状态及胃黏膜状态,增加PTEN及GSK-3β蛋白、降低PIP3蛋白表达。结论 参佛胃康可明显逆转PLGC大鼠一般情况及胃黏膜状态,其可能与增加PTEN及GSK-3β蛋白、降低PIP3蛋白的表达相关。
Objective To observe the effects and potential mechanisms of Shenfo Weikang on PTEN,GSK-3β and PIP3 in rats with precancerous lesions of gastric cancer.Methods Rats model of gastric precancerous lesions were established by MNNG comprehensive modeling method.Fifty rats were randomly divided into blank group,model group,Shenfo Weikang group and Bismuth Potassium Citrate group.Each group of rats was treated with the corresponding drug for four weeks.To observe the body weight,food intake and mental state of the rats.Hematoxylin eosin stain was used to show gastric mucosa condition.Western blot was used to detect the expression of PTEN,GSK-3β and PIP3 protein in each group.Results Shenfo Weikang can effectively improve the general and gastric mucosa stats of PLGC rats,and increase the expression of PTEN and GSK-3β protein,decrease the expression of PIP3 protein.Conclusion Shenfo Weikang can obviously improve the general and gastric mucosa stats of PLGC rats,it may be related to the increased expression of PTEN and GSK-3β protein and decreased expression of PIP3 protein.
作者
赵迪
李盟
李特
杜燕青
蔺焕萍
周小燕
ZHAO Di;LI Meng;LI Te;DU Yanqing;LIN Huanping;ZHOU Xiaoyan(Shaanxi University of Chinese Medicine,Xianyang 712046,China;No.9 hospital of Xi an,Xi an 710054,China;Huzhou Hospital of Traditional Chinese Medicine,Huzhou 313000,China;Affiliated Hospital to Shaanxi University of Chinese Medicine,Xianyang 712000,China)
出处
《陕西中医药大学学报》
2025年第3期101-106,共6页
Journal of Shaanxi University of Chinese Medicine
基金
陕西省科技厅科研项目(2022JM-465)
陕西省中医管理局中医药科研课题(JCPT004)
陕西省咸阳市科技局课题(2018KT-42)
陕西中医药大学校级科研课题(2016QN07)。
