摘要
目的 探讨肠易激综合征(IBS)患者外周血辅助性T细胞9(Th9)占比及其细胞因子白细胞介素9(IL-9)的表达水平与肠易激综合征症状严重程度量表(IBS-SSS)评分的关系。方法 选取2022年1月至2024年6月衡水市人民医院消化内科就诊的103例IBS患者作为研究组,并选取同期的103名健康人作为对照组。流式细胞术检测患者外周血中的Th9细胞占比,酶联免疫吸附试验(ELISA)法检测IL-9的表达水平。评估IBS患者的疾病程度,以IBS-SSS评分表示。采用Spearman相关性分析法分析Th9细胞占比、IL-9表达水平与IBS-SSS评分的相关性。多因素Logistic回归分析IBS发生的影响因素。采用受试者工作特征(ROC)曲线分析评估Th9细胞占比和IL-9表达水平对IBS的诊断能力。结果 研究组Th9细胞占比和血清IL-9表达水平均高于对照组(P <0.05)。中重度组的Th9细胞占比、IL-9表达水平与IBS-SSS评分均高于轻度组(P <0.05)。IBS-SSS评分与Th9细胞占比和IL-9表达水平呈正相关(P <0.05)。多因素Logistic回归分析,Th9细胞占比高和IL-9表达水平高均是患者发生IBS的风险因素。ROC曲线分析显示,Th9细胞占比和IL-9表达水平对IBS的诊断曲线下面积(AUC)为0.699(95%CI:0.584~0.813)和0.707(95%CI:0.606~0.808),二者联合对IBS的诊断AUC面积为0.851(95%CI:0.768~0.933)。结论 IBS患者外周血中Th9细胞占比和IL-9表达水平均升高,且与疾病程度呈正相关。Th9细胞占比和IL-9表达水平对IBS具有良好的诊断作用。
Objective To investigate the correlation of the proportion of T helper 9(Th9)cells and the expression level of interleukin-9(IL-9)in peripheral blood with the Irritable Bowel Syndrome Symptom Severity Scale(IBS-SSS)score in patients with irritable bowel syndrome(IBS).Methods A total of 103 IBS patients treated in the Department of Gastroenterology,Hengshui People's Hospital from January 2022 to June 2024 were enrolled in the study group,and 103 healthy people were enrolled in the control group.The proportion of Th9 cells and the expression level of IL-9 in peripheral blood were detected by flow cytometry and enzyme-linked immunosorbent assay(ELISA),respectively.The IBS severity was assessed by the IBS-SSS.The correlation of Th9 cell proportion,and IL-9 expression level with the IBS-SSS score was analyzed by Spearman correlation analysis.Factors influencing the occurrence of IBS were defined by multivariate Logistic regression analysis.Receiver operating curve(ROC)analysis was used to evaluate the value of Th9 cell proportion and IL-9 expression level in diagnosing IBS.Results The proportion of Th9 cells and serum IL-9 in the study group were significantly higher than those of the control group(P<0.05).Th9 cell proportion,IL-9 expression level and IBS-SSS score in the moderate-to-severe group were significantly higher than those of the mild group(P<0.05).The IBS-SSS score was positively correlated with the proportion of Th9 cells and the expression level of IL-9(P<0.05).Multivariate Logistic regression analysis showed that the high proportion of Th9 cells and the high level of IL-9 were both risk factors for IBS.ROC curve analysis showed that the area under the curve(AUC)of Th9 cell proportion and IL-9 expression level in diagnosing IBS was 0.699(95%CI:0.584-0.813)and 0.707(95%CI:0.606-0.808),respectively,and the AUC of their combination was 0.851(95%CI:0.768-0.933).Conclusion The proportion of Th9 cells and the expression level of IL-9 in peripheral blood of IBS patients are elevated,and they are positively correlated with the disease severity.The proportion of Th9 cells and the expression level of IL-9 have good diagnostic abilities on IBS.
作者
赵彩红
田金媚
杨彬
王丽华
苏新爱
张冰
ZHAO Caihong;TIAN Jinmei;YANG Bin;WANG Lihua;SU Xinai;ZHANG Bing(Department of Gastroenterology,Hengshui People's Hospital,Hengshui,Hebei 053000,China)
出处
《转化医学杂志》
2025年第1期80-83,145,共5页
Translational Medicine Journal
基金
衡水市2021年度科技计划项目(2021014082Z)。
