摘要
目的:评价低温等离子体(cold atmospheric plasma,CAP)对豚鼠皮肤真菌感染合并细菌定植模型的疗效。方法:35只免疫抑制豚鼠,建立红色毛癣菌、金黄色葡萄球菌复合感染模型,随机分为5组,每组7只。A组(空白组)、B组(阳性对照组,以联苯苄唑乳膏、莫匹罗星软膏处理)、C组(低剂量CAP组,30%强度即3档位)、D组(中剂量CAP组,50%强度即5档位)、E组(高剂量CAP组,80%强度即8档位)。通过临床、病原学、病理学进行疗效评估。结果:除A组外,各组治疗前后皮损评分差异均具有统计学意义(P<0.05),治疗2周后A组(11.43±0.98)分,B组(1.71±0.49)分,C组(4.86±1.07)分,D组(3.43±0.54)分,E组(1.86±1.07)分,B、C、D、E组均优于A组(P<0.001),B、D、E组间无显著差异。除A组外,各组真菌镜检、病原学培养阴性率均高于治疗前(P<0.05),组间无显著差异。B与D、E组病理学评分比较无显著差异。结论:CAP对该模型的疗效与莫匹罗星、联苯苄唑的联合作用相当,一定范围内随剂量的增大而增加,达到一定剂量后疗效维持稳定。
Objective:To evaluate the efficacy of cold atmospheric plasma(CAP)in a guinea pig skin model of fungal-bacterial co-infection.Methods:35 immunocompromised guinea pigs were used to establish a co-infection model with Trichophyton rubrum and Staphylococcus aureus.They were randomly divided into 5 groups,7 in each group.Group A(blank group),group B(positive control group,treated with bifonazole cream and mupirocin ointment),group C(low dose CAP group,30%intensity of 3 gear),group D(medium dose CAP group,50%intensity of 5 gear),group E(high dose CAP group,80%intensity of 8 gear).The efficacy was assessed through clinical,microbiological,and pathological examinations.Results:The differences in skin lesion scores before and after treatment were statistically significant(P<0.05)in 4 groups(groups B-E).After 2 weeks of treatment,group A(11.43±0.98),group B(1.71±0.49),group C(4.86±1.07),group D(3.43±0.54),and group E(1.86±1.07).Groups B,C,D,and E were better than group A(P<0.001),and there was no significant difference between group B,D and E.The negative rates of fungal microscopy and microbiological cultures in groups B-E were higher than before treatment(P<0.05),with no significant difference among groups.There were no significant difference in pathological scores between group B and D,E.Conclusion:The efficacy of CAP in this model is comparable to the combined effects of mupirocin and bifonazole,increasing with dosage within a specific range and stabilizing after reaching a certain dosage.
作者
吴晋彤
王溯
章程
黄邦斗
王睿
李承新
WU Jintong;WANG Su;ZHANG Cheng;HUANG Bangdou;WANG Rui;LI Chengxin(Medical School of Chinese PLA,Beijing 100853,China;Department of Dermatology,the First Medical Center of Chinese PLA General Hospital,Beijing 100853,China;School of Medicine,Nankai University,Tianjin 300071,China;Institute of Electrical Engineering,Chinese Academy of Sciences,Beijing 100190,China;State Key Laboratory of Kidney Diseases,Beijing 100853,China)
出处
《中国麻风皮肤病杂志》
2025年第5期337-342,共6页
China Journal of Leprosy and Skin Diseases
基金
国家自然科学基金(编号:82273530)。
关键词
低温等离子体
皮肤浅部真菌病
细菌定植
豚鼠
cold atmospheric plasma
superficial mycoses
bacterial colonization
guinea pig
