摘要
马铃薯疮痂病(potato common scab)近年来在我国主要产区广泛发生,造成严重危害,且缺乏绿色高效的防治措施。为筛选对疮痂病具有显著拮抗效果的生防菌株,本研究以疮痂链霉菌Streptomyces scabies为靶标,筛选到一株拮抗效果显著的生防菌株ZF517。通过形态学观察、生理生化特性测定以及多基因系统发育树分析,鉴定该菌株为枯草芽胞杆菌Bacillus subtilis。抑菌谱分析显示,菌株ZF517对6种病原真菌和3种病原细菌具有较高的拮抗效果。生防性状检测表明,ZF517能产生蛋白酶和嗜铁素,并含有Surfactin、Macrolactin、Fengycin、Difficidin、Bacillibactin、Bacillaene、Bacilysin和Iturin的合成基因。此外,ZF517的发酵液可抑制疮痂链霉菌菌丝的生长。经萝卜幼苗法验证,ZF517发酵液可显著抑制疮痂链霉菌对萝卜幼苗的侵染,其防效达到63.94%;盆栽试验结果表明,其对马铃薯疮痂病的防效达58.07%,显著优于对照药剂20%噻唑锌悬浮剂的防治效果。综上,菌株ZF517在防治马铃薯疮痂病方面表现出较大的生防潜力。
Potato common scab,a severe soilborne disease caused by Streptomyces scabies,has become increasingly prevalent in major potato-producing regions in China,causing significant yield and quality losses.However,effective and environmentally friendly control strategies remain limited.In this study,a biocontrol strain,ZF517,exhibiting strong antagonistic activity against S.scabies,was isolated.Based on morphological characteristics,physiological and biochemical tests,and multi-gene phylogenetic analysis,ZF517 was identified as Bacillus subtilis.Antagonistic spectrum analysis revealed that ZF517 exhibited potent inhibitory effects against six pathogenic fungi and three pathogenic bacteria.Biocontrol-related traits analysis showed that ZF517 secretes proteases and siderophores and harbours biosynthetic genes for Surfactin,Macrolactin,Fengycin,Difficidin,Bacillibactin,Bacillaene,Bacilysin,and Iturin.Moreover,the fermentation broth of ZF517 significantly inhibited the mycelial growth of S.scabies.A radish seedling assay confirmed that the fermentation broth effectively suppressed S.scabies infection,achieving a biocontrol efficacy of 63.94%.In pot experiments,ZF517 exhibited a biocontrol efficacy of 58.07%against potato common scab,significantly surpassing the efficacy of the chemical control treatment(20%thiazole zinc suspension concentrate).These findings indicate that ZF517 holds great potential as a biocontrol agent for managing potato common scab.
作者
卫鑫辰
刘易
杨茹薇
段广荣
谢学文
石延霞
柴阿丽
李宝聚
李磊
WEI Xinchen;LIU Yi;YANG Ruwei;DUAN Guangrong;XIE Xuewen;SHI Yanxia;CHAI Ali;LI Baoju;LI lei(State Key Laboratory of Vegetable Biobreeding/Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China;College of Horticultural and Landscape Architecture,Tianjin Agricultural University,Tianjin 300384,China;Xinjiang Academy of Agricultural Sciences,Comprehensive Experimental Station,Urumqi 830011,China;Sinochem Crop Protection Co.,Ltd.,Shanghai 200121,China)
出处
《中国生物防治学报》
北大核心
2025年第2期411-422,共12页
Chinese Journal of Biological Control
基金
新疆维吾尔自治区重点研发计划项目(2022B02044)
中国农业科学院科技创新工程项目(CAAS-ASTIP-IVFCAAS)
农业农村部园艺作物生物学与种质创制重点实验室(IVF2023)
蔬菜生物育种全国重点实验室开放课题。
