摘要
肠道寄生虫病是人兽共患病,不仅危害自然状态下的野生动物健康,影响野生动物种群发展,还能通过多种途径向人类传播。为了解北京市野生有蹄类动物肠道寄生虫感染情况,采集北京市云蒙山和雾灵山自然保护区狍(Capreolus pygargus)粪便样品,采用饱和食盐水漂浮法和分子生物学鉴定方法进行肠道寄生虫检测。结果显示:饱和食盐水漂浮法检测到云蒙山和雾灵山自然保护区狍肠道寄生虫阳性感染率分别为61.29%和85.71%,共检测到球虫(Coccidia sp.)、吸虫(Trematoda sp.)、细颈线虫(Nematodirus sp.)和鞭虫(Trichuris sp.)4种肠道寄生虫虫卵、卵囊或包囊,云蒙山狍感染率分别为61.29%、22.58%、12.90%和0,雾灵山狍感染率分别为76.19%、9.52%、4.76%和4.76%。感染强度分析结果显示,雾灵山狍粪便样品中肠道寄生虫虫卵、卵囊或包囊的感染强度较高。对有蹄类动物4种常见肠道寄生虫的分子鉴定显示,云蒙山和雾灵山自然保护区狍肠道寄生虫阳性总感染率分别为22.58%和19.05%,云蒙山狍普通圆线虫(Strongylus vulgaris)、毛圆属线虫(Trichostrongylus sp.)、隐孢子虫(Cryptosporidium sp.)和十二指肠贾第虫(Giardia duodenalis)的感染率分别为3.23%、16.13%、3.23%和0,雾灵山狍感染率分别为9.52%、0、0和9.52%。调查显示,北京市云蒙山和雾灵山自然保护区狍肠道寄生虫感染率较高,应及时采取有效措施,加强对野生动物肠道寄生虫病的防控,研究结果为北京市自然保护区野生动物肠道寄生虫病的防治提供了参考。
Intestinal parasitic diseases are zoonoses that not only threaten the health of wildlife and affect the development of wildlife populations,but also can be transmitted to humans through a variety of ways.In order to understand the infection situation of intestinal parasite in wild ungulates in Beijing,the intestinal parasites were detected by the saturated saline flotation method and molecular biology identification method from faecal samples of roe deer(Capreolus pygargus)in Beijing Yunmengshan and Wulingshan Nature Reserves.The results showed that the positive infection rates of roe deer intestinal parasites in Yunmengshan and Wulingshan Nature Reserves were 61.29% and 85.71%,respectively.A total of four intestinal parasites,namely Coccidia sp.,Trematoda sp.,Nematodirus sp.and Trichuris sp.were detected as eggs,oocysts or encapsulations,and the infection rates of roe deer in Yunmengshan were 61.29%,22.58%,12.90% and 0,respectively,and 76.19%,9.52%,4.76% and 4.76% in Wulingshan.The results of the infection intensity analysis showed that the fecal samples of roe deer in the Wulingshan had a high infection intensity of intestinal parasite eggs,oocysts or cysts in the fecal samples.Through molecular identification on four common intestinal parasites,the total positive infection rates were 22.58% and 19.05% in Yunmengshan and Wulingshan Nature Reserves,respectively,and that the infection rates of Strongylus vulgaris,Trichostrongylus sp.,Cryptosporidium sp.,and Giardia duodenalis were 3.23%,16.13%,3.23% and 0 in Yunmengshan,9.52%,0,0 and 9.52% in Wulingshan,respectively.The investigation found that there was a high infection rate of intestinal parasites in roe deer in the two nature reserves in Beijing.Effective measures should be taken in time to strengthen the control of intestinal parasitic diseases in wildlife in the two nature reserves.This study provides a valuable pilot work on the infection status of the intestinal parasites in wildlife in Beijing.
作者
台保倩
张梓琪
房新民
张鑫
赵建喜
伊德英
马志红
韩莹莹
高福利
鲍伟东
TAI Baoqian;ZHANG Ziqi;FANG Xinmin;ZHANG Xin;ZHAO Jianxi;YI Deying;MA Zhihong;HAN Yingying;GAO Fuli;BAO Weidong(National Engineering Research Center of Tree Breeding and Ecological Restoration,Beijing Forestry University,Beijing,100083,China;College of Biological Sciences and Biotechnology,Beijing Forestry University,Beijing,100083,China;Beijing Wulingshan Nature Reserve Administration,Beijing,101500,China;Beijing Yunmengshan Nature Reserve Administration,Beijing,101500,China)
出处
《野生动物学报》
北大核心
2025年第2期312-320,共9页
CHINESE JOURNAL OF WILDLIFE
基金
国家自然科学基金项目(32171653)。
关键词
狍
肠道寄生虫
饱和食盐水漂浮法
分子生物学鉴定
北京市
Roe deer(Capreolus pygargus)
Intestinal parasite
Saturated saline flotation method
Molecular biology identification method
Beijing
