摘要
为了探讨地下水中微生物群落的多样性及其与环境因素的关系,通过采样和分子生物学技术,研究了不同地层(第四系、白垩系、侏罗系、采空区)中微生物群落的组成及其生态环境特征。结果表明:地下水微生物群落在不同地层中存在显著差异,特别是变形菌门、放线菌门和热脱硫杆菌门的丰度变化反映了各含水层的生态环境特征。第四系样本中微生物多样性最高,而侏罗系样本则表现出显著的生态差异。此外,矿井水的抽排与通风过程引起的环境变化,显著增加了采空区微生物的多样性和生物量。基于ASV水平的聚类分析和主成分分析显示,不同水体间的微生物群落结构差异超过深度差异,提示环境组分的变化对微生物群落有重要影响。这些结果为水源判别提供了依据,并为矿区的水资源管理与环境保护策略制定提供了科学支持。深入理解地下水生态系统中微生物的响应机制,具有重要的科学价值和现实意义。
In order to explore the diversity of microbial communities in groundwater and its relationship with environmental factors,the composition and ecological environment characteristics of microbial communities in different strata(Quaternary,Cretaceous,Jurassic,goaf)were studied through sampling and molecular biology techniques.The results showed that there were significant differences in groundwater microbial communities in different strata,especially the variation of the abundance of Proteobacteria,actinobacteria and thermodesulfurization reflected the ecological environment characteristics of each aquifer.The quaternary samples had the highest microbial diversity,while the Jurassic samples showed significant ecological differences.In addition,the environmental changes caused by the drainage and ventilation of mine water significantly increased the diversity and biomass of microorganisms in the goaf.Cluster analysis and principal component analysis based on ASV level showed that the difference of microbial community structure among different water bodies exceeded the difference of depth,suggesting that the change of environmental components had an important impact on microbial community.These results provide a basis for water source identification and scientific support for water resource management and environmental protection strategy formulation in mining areas.It is of great scientific and practical significance to deeply understand the response mechanism of microorganisms in groundwater ecosystem.
作者
高国宏
阮梦颖
郝英豪
吴访
周新河
刘荣灿
李媛
GAO Guohong;RUAN Mengying;HAO Yinghao;WU Pang;ZHOU Xinhe;LIU Rongcan;LI Yuan(Geodetic and Hydrological Department of Zhongtian Hechuang Coal Branch,Ordos 017000,China;General Prospecting Institute of China National Administration of Coal Geology,Beijing 100039,China;Key Laboratory of National Mining Safety Administration for Transparent Geology and Digital Twin Technology in Mines,Beijing 100039,China)
出处
《煤炭工程》
北大核心
2024年第S1期163-170,共8页
Coal Engineering
关键词
矿区地质结构
微生物群落多样性
高通量测序
矿区水文环境
geological structure of mining areas
diversity of microbial communities
high-throughput sequencing
hydrological environment of mining areas
