摘要
从1988至1991年利用显微注射技术,向鱼类受精卵导入外源生长激素基因,获得了含鲤鱼MT启动子基因与羊生长激素基因的融合基因和含鼠MT启动子基因与牛生长激素基因的融合基因等外源基因的大批实验鱼。经斑点杂交与Southern印渍杂交分析证实,这批鱼中确有外源基因整合到基因组中的个体,比例在10%左右。生长测定显示上述转基因鲤鱼群体的变异系数远大于对照组,一些个体生长速度明显高于对照组。
Some metallothionein-growth hormone fusion genes are introduced into fertilized eggs of various fishes by mieroinjection.These fusion gemes are common carpmetallothionein-ovine growth hormone gene,common carp metallothionein-cDNA fragment of ovine growth hormone fusion genes,etc.So a big colony of transgenic fishes are available.Dot and Southern Blot analysis show that ten per cent of these transgenic fishes contain foreign genes.Some of these transgenie fishes grow much faster than controls.
作者
孙孝文
沈俊宝
王鹏
梁利群
阎学春
Sun Xiaowen;Shen Junbao;Wang Pcng;Liang Liqun;Yan xuechun(Heilongjiang Fishery Research Institute,Chenese Academy of Fishery Siences,Heilongjiang 150076)
出处
《高技术通讯》
CSCD
1992年第1期26-29,共4页
Chinese High Technology Letters
