摘要
目的建立同时测定血液中8种苯环利定(phencyclidine,PCP)类物质的超高效液相色谱-串联质谱检测方法。方法使用乙腈蛋白沉淀法处理血液,采用ACQUITY UPLC BEH C18色谱柱(2.1 mm×100 mm,1.7μm),以0.1%(体积分数)甲酸溶液-含0.1%甲酸溶液的乙腈为流动相进行梯度洗脱,流速0.4 mL/min。质谱检测采用电喷雾离子源正离子、动态多反应监测模式。结果该方法实现了对血液中8种苯环利定类物质的定性、定量分析,各目标物在0.1~50 ng/mL范围内具有良好的线性关系,相关系数均大于0.997,检出限为0.015~0.03 ng/mL,定量限为0.05~0.1 ng/mL。该方法成功应用于实际血液,于该血液中检出2-FXE成分。结论该方法具有快速、灵敏、准确、适用性广等突出特点,能够满足血液中苯环利定类物质的定性、定量分析要求,并为分析未来新出现的苯环利定类物质提供参考。
Objective To establish a determination method of ultra-high performance liquid chromatography-tandem mass spectrometry(UHPLC-MS/MS)for simultaneous analysis of 8 phencyclidine-type substances in blood.Methods Processing blood using acetonitrile protein precipitation method,use an ACQUITY UPLC BEH C18 column(2.1 mm×100 mm,1.7μm),and select acetonitrile-water containing 0.1%(volume fraction)formic acid as the mobile phase at 0.4 mL/min.Mass spectrometry was detected using an electrospray ionization-positive ion,dynamic multiple reaction monitoring(MRM)detection mode.Results This method achieved qualitative and quantitative analysis of 8 phencyclidine-type substances in blood.Each target object has a good linear relationship in the range of 0.1-50 ng/mL,with the correlation coefficients all greater than 0.997.The minimum detection limits range from 0.015 ng/mL to 0.03 ng/mL,and the minimum quantitative limits ranged from 0.05 ng/mL to 0.1 ng/mL.The proposed method was successfully applied to actual blood and detected the 2-FXE component in the blood.Conclusion This method is rapid,sensitive,accurate and has wide applicability,which can meet the requirements of qualitative and quantitative analysis of phencyclidine-type substances in blood.It can also provide a reference for analyzing new phencyclidine-type substances in the future.
作者
杨洲
石恺婷
杨荣极
李国娟
杨柳
廖彩云
徐布一
YANG Zhou;SHI Kaiting;YANG Rongji;LI Guojuan;YANG Liu;LIAO Caiyun;XU Buyi(National Anti-Drug Laboratory Sichuan Regional Center,Chengdu 610000,China;West China School of Basic Medicine Sciences&Forensic Medicine,Sichuan University,Chengdu 610041,China)
出处
《中国司法鉴定》
2025年第2期40-46,共7页
Chinese Journal of Forensic Sciences
基金
四川省科技计划项目(2023YFS0418)
毒品监测管控与禁毒关键技术公安部重点实验室开放课题(2022-KLDMC-07)。
