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Customizable PCR-based target enrichment probes for sequencing fungi-parasitized insects

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摘要 Dear Editor,Insect-fungal symbioses are fruitful testing grounds for examining coevolutionary theories,from host specificity(Stranda&Obrycki,1996;Rohrlich et al.,2018),arms-race scenarios(Pedrini et al.,2015;Stock et al.,2023),mutualisms(Aanen et al.,2002;Biedermann&Vega,2020)to biogeography(Zhang et al.,2014;Mueller et al.,2017).These investigations fundamentally depend on clarifying the systematics of both insect hosts and their fungal parasites,but the identities of many fungiparasitized insects are not well understood.This is because DNA from"zombie insect"tissues are invariably fragmented,and often overwhelmed by fungal mycelium(Fig.1,reviewed in Shang et al.,2015;Wang,&Wang,2017;Araujo&Hughes,2019).
出处 《Insect Science》 2025年第2期719-724,共6页 昆虫科学(英文版)
基金 ZW,SK,and BASM were supported by graduate fellowships from Harvard Graduate School of Arts and Sciences The development of target enrichment protocols in this study was supported by a NSF DDIG award(#1601356)to BDF and BASM.
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