摘要
目的探讨血清hsa_circRNA_0002980、hsa_circRNA_104348表达对肝癌患者诊断及预后评估的临床价值。方法选取2020年4月至2022年4月该院收治的肝癌患者及肝硬化患者各105例作为肝癌组和肝硬化组,另选择同期在该院进行体检的105例健康志愿者作为对照组。采用实时荧光定量PCR(qPCR)检测血清hsa_circRNA_0002980、hsa_circRNA_104348表达。采用受试者工作特征(ROC)曲线评估血清hsa_circRNA_0002980、hsa_circRNA_104348表达对肝癌的诊断价值及对预后的预测价值,采用多因素COX回归分析肝癌患者预后的影响因素。结果肝癌组、肝硬化组、对照组血清hsa_circRNA_0002980表达水平依次升高,血清hsa_circRNA_104348表达依次降低(P<0.05);肝癌组、肝硬化组丙氨酸氨基转移酶、天门冬氨酸氨基转移酶水平高于对照组(P<0.05),白蛋白水平低于对照组(P<0.05)。hsa_circRNA_0002980、hsa_circRNA_104348联合诊断肝癌的曲线下面积(AUC)为0.888(95%CI:0.838~0.928),大于hsa_circRNA_0002980(Z=3.526,P<0.001)及hsa_circRNA_104348(Z=2.184,P=0.029)单独诊断。预后不良组血清hsa_circRNA_0002980表达水平低于预后良好组(P<0.05),血清hsa_circRNA_104348表达水平及TNM分期为Ⅲ+Ⅳ期比例高于预后良好组(P<0.05)。hsa_circRNA_0002980、hsa_circRNA_104348联合预测肝癌患者预后的AUC为0.870(95%CI:0.790~0.928),与hsa_circRNA_0002980、hsa_circRNA_104348单独预测的AUC比较差异无统计学意义(P>0.05)。血清hsa_circRNA_0002980、hsa_circRNA_104348表达、TNM分期为肝癌患者预后的影响因素(P<0.05)。结论肝癌患者血清hsa_circRNA_0002980表达水平较低,hsa_circRNA_104348表达水平较高,二者对肝癌的诊断和预后评估有一定的临床意义。
Objective To investigate the clinical value of serum hsa_circRNA_0002980 and hsa_circRNA_104348 expression in the diagnosis and prognosis evaluation of liver cancer patients.Methods From April 2020 to April 2022,a total of 105 liver cancer patients and 105 liver cirrhosis patients admitted in the hospital were selected as the liver cancer group and cirrhosis group,and another 105 healthy volunteers who underwent physical examinations in the hospital were selected as the control group.Real-time fluorescence quantitative PCR(qPCR)was applied to detect the expression of serum hsa_circRNA_0002980 and hsa_circRNA_104348.Receiver operating characteristic(ROC)curve was applied to evaluate the diagnostic value and prognostic value of serum hsa_circRNA_0002980 and hsa_circRNA_104348 expression in liver cancer.Multivariate COX regression was performed to analyze the influencing factors of prognosis in liver cancer.Results The expression levels of serum hsa_circRNA_0002980 in the liver cancer group,liver cirrhosis group,and control group increased sequentially(P<0.05),while the expression levels of serum hsa_circRNA_104348 decreased sequentially(P<0.05).The levels of alanine aminotransferase and aspartate aminotransferase in the liver cancer group and the liver cirrhosis group were higher than those in the control group(P<0.05),and the level of albumin was lower than that in the control group(P<0.05).The area under the curve(AUC)for the diagnosis of liver cancer by hsa_circRNA_0002980 combined with hsa_circRNA_104348 was 0.888(95%CI:0.838-0.928),which was obviously higher than those of hsa_circRNA_0002980(Z=3.526,P<0.001)and hsa_circRNA_104348 alone(Z=2.184,P=0.029).The expression level of serum hsa_circRNA_0002980 in the poor prognosis group was lower than that in the good prognosis group(P<0.05),and the expression level of serum hsa_circRNA_104348 and the proportion of TNM stageⅢ+Ⅳwere higher than those in the good prognosis group(P<0.05).The AUC for predicting prognosis in liver cancer patients by the combination of hsa_circRNA_0002980 and hsa_circRNA_104348 was 0.870(95%CI:0.790-0.928),and there was no statistically significant difference compared to the AUC predicted separately by hsa_circRNA_0002980 and hsa_circRNA_104348(P>0.05).The expression of serum hsa_circRNA_0002980,hsa_circRNA_104348 and TNM stage were influencing factors for the prognosis of liver cancer patients(P<0.05).Conclusion The expression level of hsa_circRNA_0002980 in the serum of liver cancer patients is relatively low,while the expression level of hsa_circRNA_104348 is relatively high.Both have certain clinical significance in the diagnosis and prognosis evaluation of liver cancer.
作者
刘东
蔡青山
李树栋
梁家铭
LIU Dong;CAI Qingshan;LI Shudong;LIANG Jiaming(Department of Hepatobiliary Surgery,Tangshan Central Hospital,Tangshan,Hebei 063000,China)
出处
《国际检验医学杂志》
2025年第8期976-981,共6页
International Journal of Laboratory Medicine
基金
河北省中医药管理局科研计划项目(2023202)。
