摘要
本研究建立了大菱鲆(Scophthalmus maximus)鱼粉中呋喃唑酮代谢物(AOZ)残留基体标准物质的制备方法。以大菱鲆为研制对象,通过药浴方式获得大菱鲆阳性基体标准物质原料样品。原料样品通过匀浆-冻干-制粉-过筛-混匀-封装-辐照灭菌等制备工艺,获得鱼粉中AOZ残留基体标准物质候选物。经过均匀性、稳定性检验,8家实验室合作定值以及不确定度评估,确定了2种大菱鲆冻干鱼粉中AOZ的特性值分别为(7.0±0.6)μg/kg和(18.0±1.5)μg/kg(k=2)。获得的大菱鲆鱼粉基体标准物质的均匀性和稳定性良好,符合标准物质技术要求,定值结果可靠。该基体标准物质可用于水产品中AOZ残留检测,包括实验室内质量控制、实验室间能力验证及不同方法比对等工作。本方法为水产品中硝基呋喃类代谢物残留基体标准物质的制备提供了技术参考。[中国渔业质量与标准,2024,14(6):10-19]
The preparation method of matrix reference materials for furazolidone metabolite AOZ residues in turbot powder was established in this study.Taking turbot as the research object,the raw material of positive matrix reference materials containing AOZ were got by a medicated bath method.Then,the raw material samples were subjected to preparation processes such as homogenization,freeze-drying,pulverization,sieving,mixing,encapsulation,irradiation sterilization and other processes to obtain candidate of AOZ matrix reference materials of fish meal.After uniformity test and stability tests,cooperative settings by 8 laboratory and uncertainty evaluation,the quantity of AOZ in turbot powder were finally determined to be(7.0±0.6)μg/kg and(18.0±1.5)μg/kg(k=2),respectively.The obtained reference material of turbot fish meal matrix had good properties with uniformity and stability,and they could meet the technical requirements of the reference material,and the calibration results were reliable.The reference materials can be used for laboratory internal quality control,inter laboratory capability verification,and different method validation for AOZ residue detection in aquatic products.This method provides a technical reference for the preparation of residual matrix standard materials of nitrofuran metabolites in aquatic products.[Chinese Fishery Quality and Standards,2024,14(6):10-19]
作者
邢丽红
孙伟红
姚琳
孙晓杰
李兆新
XING Lihong;SUN Weihong;YAO Lin;SUN Xiaojie;LI Zhaoxin(Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Key Laboratory of Testing and Evaluation for Aquatic Product Safety and Quality,Ministry of Agriculture and Rural Affairs,Laboratory of Quality&Safety Risk Assessment for Aquatic Products(Qingdao),Ministry of Agriculture and Rural Affairs,Qingdao,266071,China)
出处
《中国渔业质量与标准》
2024年第6期10-19,共10页
Chinese Fishery Quality and Standards
基金
国家自然科学基金项目(41806148)
浙江省近岸水域生物资源开发与保护重点实验室、温州市海洋生物遗传育种重点实验室联合开放基金(J2021003)。
