摘要
【目的】鉴定织金白鹅蛋白磷酸酶3催化亚基α(PPP3CA)的单核苷酸多态性(SNPs)位点,并探究PPP3CA基因多态性对体尺性状的影响。【方法】以织金白鹅为试验对象,通过PCR扩增和Sanger直接测序法鉴定144羽织金白鹅PPP3CA基因SNPs位点,利用Excel 2010软件对基因频率、基因型频率、有效等位基因数(Ne)、多态信息含量(PIC)进行计算,分析Hardy-Weinberg平衡状态,利用SPSS 25.0软件一般线性模型(GLM)中单因素方差分析144羽织金白鹅体尺性状指标与SNPs位点间的关联性。【结果】在织金白鹅PPP3CA基因内含子2、4区域处共发现9个SNPs位点,分别为g.58398 A>G、g.58504 A>G、g.58541 A>G、g.68997 A>G、g.69001 C>T、g.69034 C>T、g.69052 T>C、g.69070 T>C和g.69114 C>T。其中,除g.69034 C>T为低度多态性位点外(PIC<0.25),其余位点均为中度多态性位点(0.25<PIC<0.5)。χ^(2)检验结果表明,位点g.58398 A>G、g.69001 C>T、g.69070 T>C和g.69114 C>T符合Hardy-Weinberg平衡(P>0.05),而位点g.58504 A>G、g.58541 A>G、g.68997 A>G、g.69034 C>T和g.69052 T>C均偏离Hardy-Weinberg平衡(P<0.05)。关联分析结果发现,在g.58504 A>G和g.58541 A>G位点中,GG基因型个体的髋骨宽极显著或显著高于AA基因型(P<0.01;P<0.05)。【结论】织金白鹅PPP3CA基因共存在9个SNPs位点,其中g.58504 A>G与g.58541 A>G位点不同基因型与髋骨宽存在不同程度相关,表明以上位点可作为织金白鹅分子标记辅助选育参考位点,为深入研究鹅PPP3CA基因奠定理论基础。
【Objective】The aim of this study was to identify the single nucleotide polymorphism(SNPs)loci of protein phosphatase 3 catalytic subunitα(PPP3CA)of Zhijin White geese,and explore the effect of PPP3CA gene polymorphism on body size traits.【Method】The PPP3CA gene SNPs of 144 Zhijin White geese were identified by PCR amplification and Sanger direct sequencing.The gene frequency,genotype frequency,number of effective allele(Ne)and polymorphism information content(PIC)were calculated using Excel 2010 software,and the Hardy-Weinberg equilibrium state was analyzed.The association between SNPs and body size traits of 144 Zhijin White geese were analyzed by One-Way analysis of variance in the general linear model(GLM)of SPSS 25.0 software.【Result】9 SNPs were found in intron 2 and 4 of PPP3CA gene in Zhijin White geese,namely g.58398 A>G,g.58504 A>G,g.58541 A>G,g.68997 A>G,g.69001 C>T,g.69034 C>T,g.69052 T>C,g.69070 T>C,and g.69114 C>T,respectively.Except for g.69034 C>T,which was a low polymorphic site(PIC<0.25),the other SNPs were moderate polymorphic loci(0.25<PIC<0.5).The Chi-square test results showed that the SNPs g.58398A>G,g.69001 C>T,g.69070 T>C and g.69114 C>T were consistent with Hardy-Weinberg equilibrium(P>0.05).However,the SNPs g.58504 A>G,g.58541 A>G,g.68997 A>G,g.69034 C>T and g.69052 T>C deviated from Hardy-Weinberg equilibrium(P<0.05).The results of association analysis showed that in the g.58504 A>G and g.58541 A>G,the pelvic width of GG genotype individuals were extremely significant or significantly higher than that of AA genotype(P<0.01 or P<0.05).【Conclusion】There were 9 SNPs in PPP3CA gene of Zhijin White geese,among which g.58504 A>G and g.58541 A>G were correlated with pelvic width to different degrees,indicating that these SNPs could be used as reference loci for molecular marker-assisted breeding of Zhijin White geese,laying a theoretical foundation for further study of PPP3CA gene in geese.
作者
赵中龙
张勇
杨红
杨润黔
艾照碧
叶丽
舒畅
ZHAO Zhonglong;ZHANG Yong;YANG Hong;YANG Runqian;AI Zhaobi;YE Li;SHU Chang(College of Animal Science,Guizhou University/Key Laboratory of Animal Genetics,Breeding and Reproduction in the Plateau Mountainous Region,Ministry of Education/Guizhou Provincial Key Laboratory of Animal Genetics,Breeding and Reproduction,Guiyang 550025,China;Bijie City Animal Husbandry Station,Bijie 551700,China)
出处
《中国畜牧兽医》
北大核心
2025年第4期1729-1738,共10页
China Animal Husbandry & Veterinary Medicine
基金
贵州省科技计划项目(黔科合支撑[2021]一般156)。
