摘要
桃蚜Myzus persicae是重要的农业害虫。本研究分析了桃蚜几丁质合成酶1(chitin synthase 1,CHS1)基因MperCHS1的序列特征,通过实时荧光定量PCR测定了MperCHS1基因在不同发育阶段的表达模式,利用病毒介导的基因沉默(virus-induced gene silencing,VIGS)技术解析了MperCHS1的生物学功能。结果表明,MperCHS1含有19个外显子,开放阅读框为4707 bp,其编码的蛋白具有保守的几丁质合成酶结构域,且与豌豆蚜CHS1亲缘关系最近。MperCHS1在1龄若虫期表达量最低,而在2龄和3龄若虫期表达量最高。沉默MperCHS1后48、72、96 h和120 h,桃蚜存活率分别下降至68.6%、57.1%、50.8%和49.2%。此外,沉默MperCHS1导致41.7%的若蚜无法正常蜕皮,雌成蚜的产蚜量也显著减少。本论文明确了MperCHS1在桃蚜生长发育和生殖中的重要功能,研究结果为开发基于MperCHS1基因沉默的桃蚜新型防治技术奠定了基础。
The green peach aphid,Myzus persicae,is an important agricultural insect pest.This study analyzed the molecular characteristics of the chitin synthase 1 gene(MperCHS1)in M.persicae.The expression pattern of MperCHS1 during different developmental stages was detected using real-time quantitative PCR.The biological function of MperCHS1 was explored via the virus-induced gene silencing(VIGS)technology.The results showed that the MperCHS1 gene consisted of 19 exons and contained an open reading frame of 4707 bp.The protein encoded by MperCHS1 had a conserved chitin synthase domain and is most closely related to CHS1 in the pea aphid,Acyrthosiphon pisum.The lowest expression level of MperCHS1 was observed in first-instar nymphs,while the highest expression occurred during second-and third-instar nymphal stages.Following MperCHS1 silencing,the survival rate of M.persicae decreased to 68.6%,57.1%,50.8%and 49.2%at 48,72,96 h,and 120 h post-treatment,respectively.Additionally,41.7%of nymphs exhibited an incomplete molting phenotype,and the reproductive output of female adults was significantly reduced.This study highlights the critical roles of MperCHS1 in the growth,development and reproduction of M.persicae,providing a foundation for developing novel pest control strategies targeting M.persicae.
作者
苗治国
洪牛
刘东阳
徐蓬军
邓全
闫芳芳
余佳敏
李茂业
刘苏
MIAO Zhiguo;HONG Niu;LIU Dongyang;XU Pengjun;DENG Quan;YAN Fangfang;YU Jiamin;LI Maoye;LIU Su(Key Laboratory of Agri-Products Quality and Biosafety,Ministry of Education,School of Plant Protection,Anhui Agricultural University,Hefei 230036,China;Liangshan Branch of Sichuan Tobacco Company,Xichang 615000,China;Tobacco Research Institute,Chinese Academy of Agricultural Sciences,Qingdao 266101,China;Panzhihua Branch of Sichuan Tobacco Company,Panzhihua 617099,China;Sichuan Institute of Tobacco Science,Sichuan Branch of China National Tobacco Corporation,Chengdu 610041,China)
出处
《植物保护》
北大核心
2025年第2期35-43,71,共10页
Plant Protection
基金
安徽省高等学校科学研究项目(2023AH051053)
安徽省教育厅优秀青年人才支持计划(gxyq2021167)
中国烟草总公司四川省公司科技项目(SCYC202410,SCYC202112)
烟草行业青年科技托举人才项目(YCQTSC202401)
烟草行业烟草病虫害监测与综合治理重点实验室开放课题(KLTPMIMT2023-09)。
关键词
桃蚜
几丁质合成酶
序列分析
表达模式
RNA干扰
Myzus persicae
chitin synthase
sequence analysis
expression pattern
RNA interference
