摘要
以六妹羊肚菌为供试菌株进行菌丝培养,分析不同生长条件(温度、pH、光照)及不同营养物质(碳源、氮源、无机盐、维生素)对羊肚菌菌丝生长长势及菌核形成的影响,旨在探寻六妹羊肚菌菌丝生长所需的最佳条件和最适宜营养物质。结果表明,供试六妹羊肚菌菌丝生长所需的最佳碳源为乳糖、氮源为酵母浸粉,最佳生长温度为25℃、pH为7、光照条件为黑暗,添加20 mg·L^(-1)的磷酸二氢钾、20 mg·L^(-1)的维生素B_(2)和10 mg·L^(-1)的维生素B_6对羊肚菌菌丝生长可起到促进作用。
Using the Morchella sextelata as the test strain,the mycelial culture method was used to analyze the effects of different growth factors(temperature,pH,light)and nutritional factors(carbon source,nitrogen source,inorganic salt,vitamin)on the mycelial growth and sclerotia formation of M.sextelata,aims to explore the best conditions and the most suitable nutrients for M.sextelata growth.The results showed that the best carbon source for the growth of M.sextelata is lactose,the best nitrogen source is yeast soaking powder,the optimal growth temperature is 25℃,the optimal pH value is 7,the optimal light condition is dark,adding 20 mg·L^(-1) of potassium dihydrogen phosphate,20 mg·L^(-1) of vitamin B_(2),10 mg·L^(-1) of vitamin B 6 can promote the growth of M.esculenta.
作者
王咪
刘跃鹏
李波
曹武红
WANG Mi;LIU Yuepeng;LI Bo;CAO Wuhong(Shanxi Agricultural University Institute of Cotton Research,Yuncheng 044000,China)
出处
《中国食用菌》
2025年第1期57-64,共8页
Edible Fungi of China
基金
运城市科学技术局科技计划项目(YCKJ-2022072)。
关键词
六妹羊肚菌
菌丝体
生长因子
碳源
氮源
无机盐
Morchella esculenta
mycelium
growth factor
carbon source
nitrogen source
inorganic salt
