摘要
【目的】探究低温条件下茶树在不同红光与远红光比值(red to far-red light ratio,R/FR)光环境中的生理与分子响应,为工厂化光设施育苗冬季补光提供理论指导。【方法】以茶树品种‘谷雨春’为材料,设置远红光处理(FR,R/FR=4.1)和白光处理(CK,R/FR=10.4),研究低温条件(10℃)下不同R/FR比值光环境对茶树气体交换参数、荧光参数及光敏色素互作因子(phytochrome interacting factors,PIFs)表达量的影响。【结果】低温条件下添加远红光、降低R/FR比值会导致茶树叶片蒸腾速率(T_(r))、净光合速率(P_(n))与电子传递速率(electron transport rate,ETR)下降,非调节性能量耗散的量子产量Y(NO)所占的比例升高,实际量子产量(YⅡ)和调节性能量耗散的量子产量Y(NPQ)所占的比例下降,叶绿素合成的重要调节因子CsPIF3a基因下调表达。【结论】低温条件下低比值的R/FR光环境削弱了茶树叶片的光合电子传递能力,抑制叶绿素合成相关基因的表达,降低光合能力,叶片受到光损伤。
【Objective】The physiological and molecular responses of tea plants under different red/far-red light ratio(R/FR)light environments under low-temperature conditions were explored to provide theoretical guidance for winter light supplementation in factory light facilities.【Method】‘Guyuchun’was used as the material,and far red light treatment(FR,R/FR=4.1)and white light treatment(CK,R/FR=10.4)were set up to study the effects of different R/FR ratio light environments on gas exchange parameters,fluorescence parameters,and the expression of phytochrome interaction factors(PIFs)of tea plants under low-temperature conditions(10℃).【Result】The transpiration rate(T_(r)),net photosynthetic rate(P_(n)),and electron transport rate(ETR)of tea leaves decreased.The proportion of non-regulated energy dissipation quantum yield Y(NO)increased,the proportion of actual quantum yield(YⅡ)and regulated energy dissipation quantum yield Y(NPQ)decreased,and the important regulator of chlorophyll synthesis CsPIF3a gene was down-regulated.【Conclusion】The low ratio of R/FR light environment under low-temperature conditions weakens the photosynthetic electron transport capacity of tea leaves,inhibited the expression of chlorophyll synthesis related genes,reduces the photosynthetic capacity,and leaves are subjected to light damage.
作者
肖富良
李解
唐敏
赖谦
翟秀明
XIAO Fuliang;LI Jie;TANG Min;LAI Qian;ZHAI Xiuming(Tea Research Institute of Chongqing Academy of Agricultural Sciences/Chongqing Engineering Technology Research Center of Tea Industry,Chongqing 402160,China;Agriculture and Rural Bureau of Gulin County,Gulin,Sichuan 646500,China)
出处
《福建农业学报》
北大核心
2025年第1期63-70,共8页
Fujian Journal of Agricultural Sciences
基金
重庆市永川区自然科学基金项目(2023yc-jckx20050)
重庆市农业科学院青年专项基金项目(KYLX20240500067)
重庆市现代农业产业技术体系“茶叶创新团队”项目(CQMAITS202308)
重庆市作物种质永川茶树资源圃项目(ZWZZ2020007)。
关键词
茶树
红光与远红光比值
光合荧光特性
光敏色素互作因子
tea plant
red to far-red light ratio
photosynthetic fluorescence characteristics
phytochrome interacting factor
