摘要
目的:探究重症肺炎患者支气管肺泡灌洗液中miR-146a与miR-149-5p的表达特征及其对预后的影响。方法:本研究纳入了2022年1月至2023年8月期间确诊的60例重症肺炎患者为观察组,依据其最终生存状态分为生存组(35例)与死亡组(25例)。同时,选取同期60例健康体检者构成对照组,比较观察组和对照组之间的miR-146a和miR-149-5p水平、不同预后患者生存组和死亡组之间的miR-146a和miR-149-5p水平之间的差异,分析患者不良预后与miR-146a和miR-149-5p表达水平之间的关联性,并探究单独检测与联合检测在效能上的差异。结果:相较于对照组,观察组患者的miR-146a水平表达显著上调,而miR-149-5p水平表达则明显下调;生存组患者的miR-146a水平显著低于死亡组,生存组患者miR-149-5p水平显著高于死亡组;患者的不良预后与miR-146a表达水平呈正相关趋势,而与miR-149-5p表达水平则呈负相关;联合检测的灵敏度显著优于单独检测,经过ROC曲线分析,不良预后患者的miR-146a、miR-149-5p临界值分别为3.09以及1.03。结论:在重症肺炎患者的支气管肺泡灌洗液中,miR-146a与miR-149-5p的表达水平对于评估患者的肺部纤维化程度及炎症反应状态具有重要提示价值。其中,患者的不良预后与miR-146a的表达呈正相关,而与miR-149-5p的表达呈负相关,可作为临床预后诊断的依据。
Objective:To investigate the expression profiles of miR-146a and miR-149-5p in bronchoalveolar lavage fluid(BALF)collected from patients with severe pneumonia and explore their correlation with patient prognosis.Methods:A cohort of 60 individuals diagnosed with severe pneumonia from January 2022 to August 2023 was established as the study's observation group.These patients were categorized into a survival group,comprising 35 cases,and a death group,consisting of 25 cases,based on their ultimate survival outcomes.Concurrently,a control group was constituted by selecting 60 healthy individuals who had routine medical check-ups during the identical timeframe.The study aimed to assess the disparities in the levels of miR-146a and miR-149-5p between the observation group versus the control group,as well as between the survival and death groups with varying patient prognoses.Additionally,the study sought to determine the relationship between the poor prognosis of patients and the expression levels of miR-146a and miR-149-5p,and to evaluate the efficacy differences between single and combined detection methods.Results:In contrast to the control group,the observation group exhibited a marked increase in miR-146a expression and a notable decrease in miR-149-5p expression.Furthermore,the survival group demonstrated significantly reduced miR-146a levels and elevated miR-149-5p levels when contrasted with the death group.The poor prognosis of patients is positively correlated with the expression level of miR-146a,while negatively correlated with the expression level of miR-149-5p;The sensitivity of joint detection is significantly better than that of individual detection.After ROC curve analysis,the critical values of miR-146a and miR-149-5p in patients with poor prognosis are 3.09 and 1.03,respectively.Conclusions:The levels of miR-146a and miR-149-5p in the bronchoalveolar lavage fluid of patients suffering from severe pneumonia are crucial biomarkers for assessing the severity of pulmonary fibrosis and the inflammatory condition.High levels of miR-146a are associated with a worse prognosis,while low levels of miR-149-5p indicate a poorer outcome.These findings can be instrumental in predicting clinical outcomes.
作者
黄海平
陈志鹏
刘勇
HUANG Haiping;CHEN Zhipeng;LIU Yong(Department of surgery ICU,Zhangzhou Hospital of Fujian Province,Zhangzhou 363000,Fujian,China)
出处
《广州医科大学学报》
2024年第6期16-20,共5页
Academic Journal of Guangzhou Medical University
