摘要
目的 探讨外周血HBV DNA、EBV DNA水平在乙型肝炎病毒(HBV)相关性肝癌合并EB病毒感染患者中的表达及临床意义。方法 采用方便抽样法选取HBV相关性肝癌合并EB病毒感染患者作为观察组,同时期选取HBV相关性肝癌但是未合并EB病毒感染患者作为对照组1,选取仅EB病毒感染患者作为对照组2。采用荧光定量PCR法检测外周血HBV DNA、EBV DNA水平,采用全自动生化分析仪检测肝功能指标水平,Spearman分析外周血HBV DNA、EBV DNA水平与肝功能指标的关系。根据观察组患者预后情况分为死亡组和生存组,比较2组外周血HBV DNA、EBV DNA水平。结果 3组患者外周血HBV DNA、EBV DNA水平比较,差异有统计学意义(P<0.05),其中观察组外周血HBV DNA、EBV DNA水平高于其他2组(P<0.05),对照组1组HBV DNA、EBV DNA水平高于对照组2(P<0.05)。3组患者肝功能指标水平比较,差异有统计学意义(P<0.05),其中观察组肝功能指标水平水平高于其他2组(P<0.05),对照组1组肝功能指标水平高于对照组2(P<0.05)。外周血HBV DNA水平与肝功能指标TBIL、IBIL无相关性(P>0.05),与ALT、AST、DBIL、ALP水平呈正相关性(P<0.05),外周血EBV DNA与肝功能指标TBIL、DBIL无相关性(P>0.05),与ALT、AST、IBIL、ALP水平呈正相关性(P<0.05)。观察组中死亡组外周血HBV DNA、EBV DNA水平均高于生存组,差异具有统计学意义(P<0.05)。结论 外周血HBV DNA、EBV DNA水平的升高与HBV相关性肝癌合并EB病毒感染的发生和预后密切相关,可能是肝癌发生和恶化的潜在标志物。
Objective To investigate the expression and clinical significance of HBV DNA and EBV DNA in peripheral blood in patients with HBV-associated liver cancer complicated with EB virus infection.Methods Using the convenient sampling method,patients with HBV-related liver cancer and EB virus infection were selected as the observation group,patients with HBV-related liver cancer but not EB virus infection were selected as the control group 1,and patients with only EB virus infection were selected as the control group 2.The levels of HBV DNA and EBV DNA in peripheral blood were detected by fluorescence quantitative PCR,and the levels of liver function indexes were detected by automatic biochemical analyzer.Spearman was used to analyze the relationship between HBV DNA,EBV DNA levels in peripheral blood and liver function indexes.According to the prognosis,the patients were divided into death group and survival group,and the levels of HBV DNA and EBV DNA in peripheral blood of the two groups were compared.Results There were statistically significant differences in the levels of HBV DNA and EBV DNA in peripheral blood among the 3 groups(P<0.05),and the levels of HBV DNA and EBV DNA in peripheral blood in the observation group were higher than those in the other 2 groups(P<0.05).The levels of HBV DNA and EBV DNA in control group 1 were higher than those in the control group 2(P<0.05).There were statistically significant differences in the levels of liver function indexes among the 3 groups(P<0.05).The level of liver function indexes in the observation group was higher than that in the other 2 groups(P<0.05),and the level of liver function indexes in the control group 1 was higher than that in the control group 2(P<0.05).The level of HBV DNA in peripheral blood was not correlated with TBIL and IBIL(P>0.05),and was positively correlated with ALT,AST,DBIL and ALP levels(P<0.05).There was no correlation between EBV DNA in peripheral blood and TBIL and DBIL(P>0.05).It was positively correlated with ALT,AST,IBIL and ALP levels(P<0.05).The levels of HBV DNA and EBV DNA in peripheral blood of the death group were higher than those of the survival group,and the difference was statistically significant(P<0.05).Conclusion The elevated levels of HBV DNA and EBV DNA in peripheral blood are closely related to the occurrence and prognosis of HBV-associated hepatocellular carcinoma complicated with Epstein-Barr virus infection,and may be a potential marker for the occurrence and deterioration of hepatocellular carcinoma.
作者
董娜
李红宇
DONG Na;LI Hongyu(Puyang Traditional Chinese Medicine Hospital,Puyang,457000)
出处
《实用癌症杂志》
2025年第3期413-417,共5页
The Practical Journal of Cancer
