摘要
为探究柠檬酸改性的羟基磷灰石纳米片(HA-Cit)对猪伪狂犬病毒(PRV)和猪传染性胃肠炎病毒(TGEV)的抑制活性及其作用方式,以CaCl_(2)、Na_(2)HPO_(4)、柠檬酸为原料,采用水热法合成HA-Cit,通过高分辨透射电镜、激光粒度仪、红外光谱仪、X射线衍射仪对HA-Cit进行图谱分析,并应用CCK-8法检测HA-Cit细胞毒性、病毒空斑法检测HA-Cit抗病毒活性及对病毒的作用方式。结果表明:相较于纯相的羟基磷灰石纳米棒(HA),HA-Cit为片状,粒径增加,拥有更强的负电性和分散性,含有柠檬酸特征官能团,结晶度降低;HACit具有更高的生物相容性,对两种病毒的治疗指数(I_(S))均显著高于HA,其抗病毒作用方式为直接灭活病毒及抑制病毒的复制和释放。
In order to explore the inhibitory activity and action mode of citric acid-modified hydroxyapatite nanosheets(HA-Cit)on porcine pseudorabies virus(PRV)and porcine infectious gastroenteritis virus(TGEV),CaCl_(2),Na_(2)HPO_(4) and citric acid were used as raw materials to prepare HA-Cit by hydrothermal method,and HA-Cit was characterized by high-resolution transmission electron microscopy,laser particle size analyzer,infrared spectrometer and X-ray diffractometer.Subsequently,the cytotoxicity of HA-Cit was detected by CCK-8 method,and the antiviral activity and action mode on viruses were detected by viral plaque method.The results showed that compared with the pure phase hydroxyapatite nanorods(HA),HA-Cit was flaky,with increased particle size,higher electronegative and dispersible properties,and contained characteristic functional groups of citric acid,with reduced crystallinity.Furthermore,HA-Cit had better biocompatibility and the selection index(I_(S))of both viruses was significantly higher than that of HA,and its antiviral mode was to directly inactivate the virus and inhibit the replication and release of the virus.
作者
黄正群
谭辉容
赵兴洪
万红平
HUANG Zhengqun;TAN Huirong;ZHAO Xinghong;WAN Hongping(Central Laboratory for Animal Infectious Disease Control,College of Veterinary Medicine,Sichuan Agricultural University,Chengdu 611130,China;Sichuan Provincial Key Laboratory of Animal Diseases and Human Health,Sichuan Agricultural University,Chengdu 611130,China)
出处
《东北农业大学学报》
CSCD
北大核心
2024年第8期142-150,共9页
Journal of Northeast Agricultural University
基金
国家自然科学基金项目(2122219061)
中央引导地方科技发展专项(2212129410)。
关键词
柠檬酸
羟基磷灰石
抗病毒活性
伪狂犬病毒
传染性胃肠炎病毒
citric acid
hydroxyapatite
antiviral activity
pseudorabies virus
transmissible gastroenteritis virus
