摘要
目的:探讨不同移植方式下人脱落乳牙牙髓干细胞(SHED)对Wistar-Kyoto(WKY)抑郁模型大鼠的治疗作用。方法:将30只雄性WKY大鼠随机分为模型组、脑室注射组、经鼻递送组,每组10只;另有10只雄性Wistar大鼠作为对照组。WKY大鼠适应性饲养1周后分别给予脑室内注射SHED细胞和鼻内递送SHED细胞,移植3周后通过蔗糖偏好实验、Y迷宫实验、强迫游泳实验及旷场实验评估不同移植方式下SHED治疗的效果;酶联免疫吸附试验(ELISA)检测神经营养因子BDNF和炎症相关因子IL-1β、IL-6、IL-10及TNF-α等的表达水平。结果:行为学实验观察到SHED移植治疗可以减轻WKY抑郁模型大鼠的抑郁样行为并提高大鼠短期记忆力,脑室注射组的治疗作用高于经鼻递送组;ELISA试验检测到SHED移植治疗后大鼠海马区和血清中神经营养因子BDNF和抗炎因子IL-10表达水平升高,而促炎因子IL-1β、IL-6及TNF-α等的表达水平降低,脑室注射组的治疗作用高于经鼻递送组。结论:SHED移植治疗具有抗抑郁作用,可能是通过神经营养保护和免疫调节抗炎的方式实现,脑室内移植治疗的作用优于经鼻递送。
Objective:To investigate the therapeutic effects of stem cells from human exfoliated deciduous teeth(SHED)transplanted by different methods on the Wistar-Kyoto(WKY)depression model rats.Methods:30 male WKY rats were randomly divided into three groups:the model group,intraventricular injection group,and intranasal delivery group,with 10 rats in each group.10 male Wistar rats served as the control group.The WKY rats was acclimated for 1 week before receiving either intraventricular injection or intranasal delivery of SHED cells.3 weeks after transplantation,the therapeutic effects of SHED transplantation were evaluated using sucrose preference test,Y-maze experiment,forced swimming test,and open field test.Additionally,enzyme-linked immunosorbent assay(ELISA)was used to detect the expression levels of neurotrophic factor BDNF and inflammation-related factors IL-1β,IL-6,IL-10,and TNF-α.Results:Behavioral experiments demonstrated that SHED transplantation alleviated depressive-like behaviors and improved short-term memory in WKY depression model rats.The therapeutic effect of intraventricular injection was superior to intranasal delivery.ELISA experiments revealed increased expression of neurotrophic factor BDNF and anti-inflammatory factor IL-10 in the hippocampus and serum following SHED transplantation;meanwhile pro-inflammatory factors IL-1β,IL-6,and TNF-αshowed decreased expression.Again intraventricular injection exhibited a more pronounced therapeutic effect compared to intranasal delivery.Conclusion:SHED transplantation therapy has an antidepressant effect possibly achieved through neural nutritional protection and immune regulation against inflammation pathways,and intraventricular transplantation appears more effective than intranasal delivery.
作者
于菲
许成岗
刘莎
徐勇
YU Fei;XU Chenggang;LIU Sha(Department of Clinical Psychology,Heping Hospital Affiliated to Changzhi Medical College,Changzhi City,Shanxi Province 046000;不详)
出处
《医学理论与实践》
2025年第6期901-905,共5页
The Journal of Medical Theory and Practice
基金
长治医学院附属和平医院2020年度院级科研基金(HPYJ202039)
国家自然科学基金面上项目(82371511)。
关键词
人脱落乳牙牙髓干细胞
重度抑郁症
WKY抑郁模型大鼠
脑立体定位注射移植
经鼻递送
Stem cells from human exfoliated deciduous teeth
Major depressive disorder
WKY depression model rats
Intraventricular injection
Intranasal delivery
