摘要
以实验室保藏的纳豆芽孢杆菌SIPI-W-N5为出发菌株,通过紫外诱变和DES诱变,获得了突变株SIPI-W-N5-16,采用响应面优化试验对其发酵培养基进行优化。结果表明,突变株SIPI-W-N5-16较出发菌株产纳豆激酶活力提高了149.24%,并确定了该突变株最佳摇瓶发酵配方,采用该发酵培养基发酵突变株SIPI-W-N5-16,其产纳豆激酶活力较优化前提高了104%。
Using a Bacillus subtilis SIPI-W-N5 strain preserved under laboratory conditions as the starting strain,a mutant strain SIPI-W-N5-16 was obtained through ultraviolet(UV)and diethyl sulfate(DES)mutagenesis.Response surface methodology(RSM)experiments were conducted to optimize the fermentation medium for the mutant strain.The results showed that the nattokinase activity of SIPI-W-N5-16 was 149.24% higher than that of the original strain.The optimal fermentation medium for shake flask cultivation of the mutant strain was determined.Fermentation of SIPI-W-N5-16 with the optimized medium led to a 104% enhancement in nattokinase activity compared to pre-optimization conditions.
作者
文雯
胡海峰
WEN Wen;HU Haifeng(Yangling Vocational&Technical College,Yangling,Shaanxi 712100,China;China Pharmaceutical University,Nanjing,Jiangsu 211100,China;Sinopharm Health Industry Institute Co.,Ltd.,Shanghai 201203,China)
出处
《陕西农业科学》
2025年第2期9-16,共8页
Shaanxi Journal of Agricultural Sciences
基金
陕西省重点研发计划项目(2023-YBNY-249)
陕西省教育厅青年创新团队建设项目(21JP143)
杨凌职业技术学院2023年校内科研基金项目(DT2023-001)。
关键词
纳豆激酶
发酵
菌株选育
工艺优化
响应面试验
Nattokinase
Fermentation
Strain breeding
Process optimization
Response surface methodology
