摘要
目的:验证本实验室是否具备食品中克罗诺杆菌属(阪崎肠杆菌)的分离鉴定以及定量分析能力,为今后食品安全风险监测过程中的定量测试提供有效的质量保证。方法:按照测量审核参试指导书处理样品,依据《食品安全国家标准食品微生物学检验克罗诺杆菌属(阪崎肠杆菌)检验》(GB 4789.40—2016)规范操作。结果:第一次克罗诺杆菌属(阪崎肠杆菌)定量检测质控考核结果不满意,第二次补测结果满意。结论:本实验室具备克罗诺杆菌属(阪崎肠杆菌)的分离鉴定和定量分析能力。实验过程中的试剂比对、人员比对、方法比对、质量控制是影响定量结果的关键因素,定性是定量结果准确的前提,将质量控制和质量监管贯穿整个实验活动,可确保微生物的检验质量。
Objective:To verify whether our laboratory has the ability of isolation,identification and quantitative analysis of Cronobacter(Enterobacter sakazakii)in food,so as to provide effective quality assurance for quantitative tests in the future food safety risk monitoring process.Method:The samples were processed according to the test instructions of measurement and audit,and were operated according to GB 4789.40—2016.Result:The results of the first Cronobacter(E.sakazakii)quantitative test were not satisfactory,and the second retest was satisfactory.Conclusion:Our laboratory is capable of isolation,identification and quantitative analysis of Cronobacter(E.sakazakii).Reagent comparison,personnel comparison,method comparison and quality control in the experiment process are the key factors affecting the quantitative results,and qualitative is the premise of accurate quantitative results.Quality control and quality supervision throughout the entire experimental activity can ensure the quality of microbial inspection.
作者
聂玉敏
李嘉蕊
段云权
李存仙
吴学林
李成
NIE Yumin;LI Jiarui;DUAN Yunquan;LI Cunxian;WU Xuelin;LI Cheng(Chuxiong Yi Autonomous Prefecture Center for Disease Control and Prevention Testing Center,Chuxiong 675000,China)
出处
《食品安全导刊》
2025年第8期52-55,共4页
China Food Safety Magazine
关键词
模拟食品
克罗诺杆菌属
定量测试
能力验证
simulated food
Cronobacter
quantitative testing
capability verification
