摘要
利用高通量测序技术,分析UV-B(280~320nm)辐射增强对苦荞叶内生菌多样性、群落组成及功能的影响,以探讨“苦荞-内生菌”互作系统在UV-B辐射胁迫下的生态适应性,为相关研究奠定理论基础。以云荞2号为研究对象,自然光作为对照(CK),设置2个UV-B辐射强度(2.5、5.0kJ·m^(-2)),处理30d后,通过高通量测序技术分析在UV-B辐射增强条件下苦荞叶内生菌的多样性、群落组成及生物学功能变化。结果表明,在97%的序列相似性水平上,所有测序样本中苦荞叶内生真菌与内生细菌分别获得167个和425个操作分类单元(OTU)。UV-B辐射处理降低了苦荞叶内生真菌与内生细菌的总OTU数量,表现为CK>5.0kJ·m^(-2)>2.5kJ·m^(-2),但对内生菌的多样性及丰富度无显著影响(P>0.05)。3个处理组的内生真菌均以子囊菌门(Ascomycota)为优势真菌门,其相对丰度随UV-B辐射增强而升高;共有的优势细菌门为变形菌门(Proteobacteria)与厚壁菌门(Firmicutes),共有的优势细菌属为未分类产碱杆菌科(unclassified_f_Alcaligenaceae)与假单胞菌属(Pseudomonas),其相对丰度随UV-B辐射增强而显著升高。单因素相关性网络分析表明,增强UV-B辐射可促进苦荞叶内生细菌群落间的协同共生作用、削弱种间竞争,同时减弱内生真菌群落间的相互影响。对内生菌进行功能预测与分析,发现UV-B辐射增强可导致植物病原菌类功能的真菌相对丰度下降,而内生细菌功能基因中参与细胞壁/细胞膜结构的生物合成、脂质转运和代谢以及蛋白质翻译后修饰等相关基因的丰度有所上升。研究表明,UV-B辐射增强导致苦荞叶内生细菌群落组成与生物学功能发生明显改变,优势细菌群种类增加、菌群间的协同共生作用增强,这有助于提升苦荞对于UV-B辐射增强的生态适应性。
This study aims to explore the mutualistic relationship between tartary buckwheat and endophytes in their ecological adaptation to UV-B radiation stress,thereby establishing a theoretical foundation for related research.Using high-throughput sequencing technology,we analyzed the effects of enhanced UV-B(280–320 nm)radiation on the diversity,community composition,and function of endophytes in tartary buckwheat leaves.The research was conducted using Yunqiao No.2 as the subject.Natural light served as the control(CK),while two UV-B radiation intensities(2.5 and 5.0 kJ·m^(-2))were applied over a period of 30 days.The diversity,community composition,and biological functions of endophytes in tartary buckwheat leaves were analyzed through high-throughput sequencing under enhanced UV-B radiation conditions.The results indicated that at a 97%sequence similarity level,167 OTUs were identified among all sequenced samples of tartary buckwheat leaf endophytes,while 425 OTUs were identified for endophytic bacteria.The UV-B radiation treatment resulted in a reduction in the total number of OTUs for both tartary buckwheat leaf endophytes and endophytic bacteria,demonstrating the following trend:CK>5.0 kJ·m^(-2)>2.5 kJ·m^(-2).However,there was no significant effect on the diversity and abundance of endophytes(P>0.05).In all three treatment groups,the endophytic fungi were predominantly Ascomycota,with their relative abundance increasing alongside enhanced UV-B radiation.The dominant bacterial phyla included Proteobacteria and Fusobacteria,with Proteobacteria and Firmicutes being the common dominant phyla.The prevalent bacterial genera were unclassified_f_Alcaligenaceae and Pseudomonas,both of which showed a significant increase in relative abundance with enhanced UV-B radiation.One-way correlation network analysis revealed that increased UV-B radiation could promote synergistic symbiosis among endophytic bacterial communities in tartary buckwheat leaves,while weakening interspecific competition and interactions among endophytic fungal communities.Functional prediction and analysis of endophytic bacteria indicated that enhanced UV-B radiation led to a decrease in the relative abundance of fungal phytopathogenic species.Conversely,there was an increase in the abundance of endophytic bacterial genes associated with cell wall/cell membrane structure,lipid transport and metabolism,post-translational modification of proteins,and other related functions.Enhanced UV-B radiation resulted in significant changes in the composition and biological functions of endophytic bacterial communities in tartary buckwheat leaves,characterized by an increase in the number of dominant bacterial species and a heightened synergistic role among these communities.This may contribute to improving the ecological adaptability of tartary buckwheat to increased UV-B radiation.
作者
肖欢
杨伟亮
孔祺
龚润林
段玉莲
赵银紫
罗宗龙
冯源
leaves XIAO Huan;YANG Weiliang;KONG Qi;GONG Runlin;DUAN Yulian;ZHAO Yinzi;LUO Zonglong;FENG Yuan(College of Agriculture and Biological Sciences,Dali University,Dali 671000,China;College of Agriculture and Biological Sciences,Dali University,Key Laboratory of Agricultural Ecology of Erhai Lake Basin,Yunnan Provincial Department of Education,Dali 671000,China;College of Agriculture and Biological Sciences,Dali University,Yunnan Provincial Collaborative Innovation Center for Integrated Protection of Cangshan Mountain and Erhai Lake and Green Development of Basin,Dali 671000,China)
出处
《农业环境科学学报》
北大核心
2025年第2期320-329,共10页
Journal of Agro-Environment Science
基金
云南省科技厅科技计划项目-地方高校联合专项(202001BA070001-210)。
关键词
UV-B辐射
苦荞
高通量测序
内生菌
微生物多样性
UV-B radiation
tartary buckwheat
high-throughput sequencing
endophyte
microbial diversity
