摘要
为给马尾松(Pinus massoniana)SAUR(Small Auxin-up RNA)基因功能解析提供参考,分析其基本特征及其在马尾松侧枝发育中的作用,基于课题组前期获得的马尾松转录组数据,对马尾松中的SAUR基因进行筛选,从系统发育及蛋白理化性质、功能结构、保守基序和保守结构域等方面对PmSAUR基因进行生物信息学分析,并采用qRT-PCR对PmSAUR基因响应GR24、6-BA、TIS-108和去顶梢处理的表达模式进行分析。结果表明,从马尾松转录组数据中共鉴定出32个SAUR基因,为PmSAUR1~PmSAUR32;PmSAUR蛋白编码113~234个氨基酸,蛋白分子量为12850.98~26563.57 Da,蛋白脂溶性系数为70.92~116.82,87.50%的PmSAUR蛋白理论等电点为偏碱性,87.50%的PmSAUR蛋白为不稳定蛋白,所有PmSAUR蛋白均为亲水蛋白。PmSAUR蛋白均存在α螺旋、延伸链、β转角和无规则卷曲,以α螺旋和无规则卷曲为主;PmSAUR蛋白亚细胞定位较分散,主要分布在叶绿体、线粒体、细胞质和细胞核上。Motif1(RFVIPTSYLNHPLFRALLEKA)高度保守,可作为鉴定PmSAUR基因的重要依据。PmSAUR7、PmSAUR11、PmSAUR21和PmSAUR32基因在6-BA处理第7天时均具有组织表达特异性,在针叶中均为高表达,在嫩茎中均为低表达;通过对4个PmSAUR基因的表达模式进行分析,推测PmSAUR7基因可能是影响马尾松侧枝发育的关键基因。
In order to provide references for functional analysis on SAUR genes in Pinus massoniana,and analyze their basic characteristics and roles in lateral branch developments of P.massoniana,based on P.massoniana transcriptome data obtained by research group at previous stage,SAUR genes in P.massoniana were screened.Bioinformatics of PmSAUR genes were analyzed from phylogenetic,physical and chemical characteristics of proteins,functional structures of proteins,conserved motifs of proteins,and conserved structural domains of proteins.Expression patterns of PmSAUR genes on responses to GR24,6-BA,TIS-108 and apical bud removal treatments were analyzed by qRT-PCR.Results showed that a total of 32 SAUR genes(PmSAUR1-PmSAUR32)were identified from P.massoniana transcriptome data.PmSAUR proteins encoded 113-234 amino acids.Protein molecular weights ranged from 12850.98 to 26563.57 Da.Protein aliphatic indexes ranged from 70.92 to 116.82.87.50%of PmSAUR proteins had alkaline theoretical isoelectric points,87.50%of PmSAUR proteins were instable proteins,and all PmSAUR proteins were hydrophilic proteins.All PmSAUR proteins had alpha helixes,extended strands,beta turns and random coils,dominating by alpha helixes and random coils.Subcellular localization of PmSAUR proteins were dispersal,mainly distributing in chloroplasts,mitochondria,cytoplasm and nuclei.Motif1(RFVIPTSYLNHPLFRALLEKA)was highly conservative,which could be important foundation for identifying PmSAUR genes.PmSAUR7,PmSAUR11,PmSAUR21 and PmSAUR32 had tissue expression specificities on 7 d of 6-BA treatment,and all of them were highly expressed in needles and lowly expressed in stems.Through analysis of expression patterns of four PmSAUR genes,it was hypothesized that PmSAUR7 might be key gene to effect P.massoniana lateral branch developments.
作者
姚筱敏
胡颖
陈虎
杨章旗
Yao Xiaomin;Hu Ying;Chen Hu;Yang Zhangqi(College of Forestry,Guangxi University,Key Laboratory of National Forestry and Grassland Administration on Cultivation of Fast Growing Timber in Central South China,Guangxi Key Laboratory of Forest Ecology and Conservation,Nanning,Guangxi 530004,China;Guangxi Forestry Research Institute,Key Laboratory of National Forestry and Grassland Administration on Cultivation of Fast-Growing Timber in Central South China,Engineering Technology Research Center of Masson Pine of National Forestry and Grassland Administration,Nanning,Guangxi 530002,China)
出处
《广西林业科学》
2025年第1期1-12,共12页
Guangxi Forestry Science
基金
国家自然科学基金项目(32160382)
广西八桂学者专项(2019A26)
广西自然科学基金项目(2019GXNSFBA245064)。
关键词
SAUR基因
生物信息学
侧枝发育
表达模式
马尾松
SAUR gene
bioinformatics
lateral branch development
expression pattern
Pinus massoniana
