摘要
目的:建立HPLC法同时测定儿童患者血清中万古霉素、咖啡因和氨茶碱浓度的方法并应用于临床检测。方法:选用6%高氯酸为蛋白沉淀剂,乙酰苯胺为内标物,采用Nucifera C_(18)(250 mm×4.6 mm,5μm)色谱柱,以甲醇-0.05 mol·L^(-1)磷酸二氢钾溶液(18∶82,v/v)为流动相,流速1 mL·min^(-1),检测波长236 nm,柱温25℃,进样量20μL。结果:万古霉素和氨茶碱血清药物浓度在1.0~100μg·m L^(-1)范围内线性关系良好,标准曲线分别为Y=0.0106X+0.0069(r=0.9988)、Y=0.0127X+0.0084(r=0.9989);咖啡因血清药物浓度在1.0~160μg·m L^(-1)范围内线性关系良好,标准曲线为Y=0.0154X-0.0101(r=0.9991);定量限均为1.0μg·m L^(-1);待测物的平均相对回收率均在97.8%~103.1%,日内和日间的精密度相对标准偏差均<10%,样品稳定性良好。收集10例接受万古霉素、氨茶碱或咖啡因治疗的患儿进行方法验证,符合临床检测需求。结论:本方法操作简便,准确度高,适应于儿童患者血清万古霉素、氨茶碱或咖啡因浓度的同时测定。
Objective:To establish an HPLC method for simultaneous determination of vancomycin,caffeine and aminophylline in serum of children patients and apply it to clinical detection.Methods:6%perchloric acid was used as protein precipitator and acetanilide was used as internal standard.The determination was performed on Nucifera C_(18)column(250 mm×4.6 mm,5μm)with mobile phase methanol-0.05 mol·L^(-1)potassium dihydrogen phosphate(18∶82,v/v).The detection wavelength was 236 nm,the flow rate was 1 mL·min^(-1),the column temperature was 25℃,and the sample size was 20μL.Results:The serum concentrations of vancomycin and aminophylline were in the range of 1.0-100μg·mL^(-1),and the standard curves were Y=0.0106X+0.0069(r=0.9988),Y=0.0127X+0.0084(r=0.9989),respectively.The serum concentration of caffeine had a good linear relationship in the range of 1.0-160μg·mL^(-1),and the standard curve was Y=0.0154X-0.0101(r=0.9991).Quantitative downlines were all 1.0μg·mL^(-1).The average recovery rates of the three compounds ranged from 97.8%to 103.1%,and the relative standard deviations of intraday and daytime precision were less than 10%,indicating good sample stability.Ten cases of children receiving vancomycin,aminophylline or caffeine were collected for method verification,which met the needs of clinical detection.Conclusion:The method is simple,rapid,accurate,and is suitable for the simultaneous determination of serum vancomycin,caffeine and aminophylline in children.
作者
李赛
李寿林
王东亮
苏凌璎
LI Sai;LI Shou-lin;WANG Dong-liang;SU Ling-ying(Department of Pharmacy,Huai'an Women and Children's Health Care Hospital,Huai'an 223001,China;The Affiliated Huai'an No.1 People's Hospital of Nanjing Medical University,Huai'an 223300,China)
出处
《药物分析杂志》
北大核心
2025年第1期92-98,共7页
Chinese Journal of Pharmaceutical Analysis
基金
淮安卫生健康科研项目(HAWJ201922)。
关键词
高效液相色谱法
万古霉素
咖啡因
氨茶碱
儿童患者
血药浓度
同时测定
high performance liquid chromatography
vancomycin
caffeine
aminophylline
pediatric patients
serum concentration
simultaneous determination
