摘要
旨在探究3β羟基类固醇脱氢酶(3β-hydroxysteroid dehydrogenase,3BHSD)基因单核苷酸多态性(single nucleotide polymorphism,SNP)及其与繁殖性状的相关性。以500只黑头山羊母羊为研究对象,采集颈静脉血提取基因组DNA,利用全基因组重测序技术筛选山羊3BHSD基因SNPs位点,分析3BHSD基因的遗传多样性,并与山羊头胎、第二胎、第三胎和平均胎次产羔数,以及羔羊初生重进行关联分析。结果:共筛选到与产羔数相关的SNPs位点5个(g.96842543C>T、g.96846699T>C、g.96846704T>C、g.96847162T>C、g.96848096C>T),与初生重相关的SNPs位点4个(g.96841899C>T、g.96844274A>G、g.96844478G>A、g.96846360T>A),上述全部SNPs位点均符合哈代-温伯格(Hardy-Weinberg)平衡状态(P>0.05);关联分析显示,g.96842543C>T及g.96846704T>C位点与山羊头胎产羔数显著相关,g.96846699T>C位点与二胎产羔数显著相关,g.96847162T>C位点与头胎、三胎及平均胎次产羔数显著相关,g.96848096C>T位点与二胎及平均胎次产羔数显著相关;连锁不平衡结果显示,g.96842543C>T、g.96846704T>C及g.96847162T>C,g.96841899C>T与g.96844478G>A处于紧密连锁不平衡,但组合基因型间与性状关联分析差异不显著。本研究筛选出3BHSD基因中5个SNPs位点与山羊产羔数显著相关,4个SNPs位点与初生重显著相关,上述SNPs位点可作为山羊繁殖性状遗传改良的分子标记。
In order to explore the single nucleotide polymorphisms(SNP)of 3β-hydroxysteroid dehydrogenase(3BHSD)gene and its correlation with reproductive traits,a total of 500 Black Head goats were selected as subjects.Cervical venous blood samples were collected from the goats and DNA was extracted.The SNPs of 3BHSD gene were screened using the whole genome resequencing technology,the genetic diversity of 3BHSD gene was analyzed,and the correlations between 3BHSD gene SNPs and the first,second,third litters,the average litter size and birth weight of lambs were analyzed.The results showed that five SNPs loci related to litter size(g.96842543C>T,g.96846699T>C,g.96846704T>C,g.96847162T>C,and g.96848096C>T),and four SNPs loci related to birth weight(g.96841899C>T,g.96844274A>G,g.96844478G>A,and g.96846360T>A)were screened in the goat population.The Chi-square fitness test results showed that all the above 9 SNPs loci were in line with the Hardy-Weinberg equilibrium(P>0.05).The correlation analysis results showed that g.96842543C>T and g.96846704T>C were significantly correlated with the first litter size;g.96846699T>C was significantly correlated with the second litter size;and g.96847162T>C the first,third,and average litter sizes;and g.96847162T>C was significantly correlated with the second and average litter sizes.The Linkage disequilibrium results showed that g.96842543C>T,g.96846704T>C and g.96847162T>C were in strong linkage disequilibrium;and g.96841899C>T and g.96844478G>A were in strong chain imbalance;but there was no significant difference between the combined genotypes and the correlation analysis of traits.In conclusion,five SNPs loci of 3BHSD gene were significantly correlated with the quantity of lamb births,and four SNPs loci were significantly correlated with birth weight;these SNPs loci might be used as molecular markers for genetic improvement of reproductive traits in goats.
作者
张凤
廖正军
杨晓培
杨务军
刘建霞
马开宇
张年
熊琪
ZHANG Feng;LIAO Zhengjun;YANG Xiaopei;YANG Wujun;LIU Jianxia;MA Kaiyu;ZHANG Nian;XIONG Qi(Institute of Animal Sciences and Veterinary Medicine,Hubei Academy of Agricultural Sciences/Key Laboratory of Animal Embryo Engineering and Molecular Breeding of Hubei Province,Wuhan 430064,China;Yunxi County Animal Husbandry Technology Extension Station,Yunxi 442600,China;Wuhan Animal Disease Prevention and Control Center,Wuhan 430014,China;Yunxi County Animal Husbandry and Veterinary Service Center,Yunxi 442600,China;Wuhan Agricultural School,Wuhan 430043,China;College of Animal Science and Technology,Huazhong Agricultural University,Wuhan 430070,China)
出处
《畜牧与兽医》
北大核心
2025年第3期11-18,共8页
Animal Husbandry & Veterinary Medicine
基金
湖北省尖刀项目(2023BAA029)
湖北省支持种业高质量发展资金项目(HBZY2023B008)
湖北省重点研发计划项目(2023BBB132)。
