摘要
目的 探讨甲基转移酶样蛋白(METTL)14检测在冠心病(CHD)患者中的临床价值。方法选取2019年6月—2021年2月河南省人民医院以胸闷或胸痛为主诉,为明确诊断完善一系列临床检查后需行冠状动脉造影明确冠状动脉狭窄情况的患者316例。根据冠状动脉造影结果,将其分为CHD组(216例)和对照组(100例)。收集所有研究对象临床资料,并采用酶联免疫吸附试验检测血浆METTL14、肿瘤坏死因子-α(TNF-α)、单核细胞趋化蛋白-1(MCP-1)、血管细胞黏附分子-1(VCAM-1)、细胞间黏附分子-1(ICAM-1)和白细胞介素(IL)-6水平。采用Pearson或Spearman分析评估各项目之间的相关性。采用受试者工作特征(ROC)曲线评估METTL14诊断CHD的效能。采用Logistic回归分析评估CHD发生的危险因素。结果 与对照组相比,CHD组TNF-α、MCP-1、VCAM-1、ICAM-1、IL-6和METTL14水平显著升高(P<0.001)。不同冠状动脉病变支数各组C-反应蛋白(CRP)和METTL14水平差异有统计学意义(P<0.05)。CHD组METTL14与IL-6和Gensini评分呈正相关(r值分别为0.390、0.493,P<0.05)。MCP-1、VCAM-1、IL-6、METTL14水平升高是CHD发生的危险因素(P<0.001)。结论 外周血METTL14水平与CHD发生有关联,是CHD的危险因素之一。
Objective To investigate the role of methyltransferase-like protein(METTL)14 in coronary heart disease(CHD)patients.Methods From June 2019 to February 2021,316 patients in Henan Provincial People's Hospital with chest tightness or chest pain as the main complaint were enrolled to undergo coronary angiography to confirm coronary artery stenosis after a series of clinical examinations.According to the results of coronary angiography,they were classified into CHD group(216 cases)and control group(100 cases).The clinical data were collected.Plasma levels of METTL14,tumor necrosis factor-alpha(TNF-α),monocyte chemotactic protein-1(MCP-1),vascular cell adhesion molecule-1(VCAM-1),intercellular adhesion molecule-1(ICAM-1)and interleukin(IL)-6 were determined by enzyme-linked immunosorbent assay.Correlations were assessed by Pearson or Spearman analysis.The effectiveness of METTL14 in diagnosing CHD was evaluated using receiver operating characteristic(ROC)curves.The risk factors of CHD were evaluated by Logistic regression analysis.Results Compared with control group,the levels of TNF-α,MCP-1,VCAM-1,ICAM-1,IL-6 and METTL14 in CHD group were increased(P<0.001).There was statistical significance in C-reactive protein(CRP)and METTL14 among all coronary lesion number groups(P<0.05).METTL14 was positively related with IL-6 and Gensini scores in CHD group(r=0.390 and 0.493,P<0.05).Increased MCP-1,VCAM-1,IL-6 and METTL14 were risk factors for CHD(P<0.001).Conclusions METTL14 in peripheral blood is related with CHD,which can be a risk factor for the diagnosis of CHD.
作者
郭凤霞
胡冰
沙燕华
GUO Fengxia;HU Bing;SHA Yanhua(Department of Clinical Laboratory,Henan Provincial People's Hospital,Zhengzhou 450003,Henan,China;Department of Clinical Laboratory,the Affiliated Cancer Hospital of Zhengzhou University,Zhengzhou 450003,Henan,China;Department of Clinical Laboratory,the Second Affiliated Hospital of Guangzhou University of Chinese Medicine,Guangzhou 510120,Guangdong,China)
出处
《检验医学》
2025年第1期54-58,共5页
Laboratory Medicine
基金
国家自然科学基金项目(82002199)
河南省医学科技攻关项目(LHGJ20230009)
河南省中青年卫生科技创新项目(YQRC2023017)
河南省科技攻关项目(232102310084)。
关键词
甲基转移酶样蛋白14
冠心病
炎性生物标志物
Methyltransferase-like protein 14
Coronary heart disease
Inflammatory biomarker
