摘要
为建立气相色谱串联质谱法(GC-MS)测定各类食品基质中双丙酚丁的分析方法,样品用乙酸乙酯进行液液萃取,PSA净化,采用HP-5MS色谱柱分离,EI源SIM模式测定,以317 m/z为定量离子,288、373、429 m/z为定性离子,外标法定量。双丙酚丁在0.050~2.00 mg·L^(-1)范围内线性良好,相关系数r2为0.9999,在加标浓度分别为0.010、0.050、0.100 mg·kg^(-1)(n=6)时,压片糖果、果冻、蜜饯和饮料等4种食品类别的回收率为80.6%~90.5%,RSD为3.4%~4.6%,检出限(S/N=3)为0.003 mg·kg^(-1),定量限(S/N=10)为0.010 mg·kg^(-1)。研究表明,该方法稳定准确,重复性好、灵敏度高,适用于各类食品中双丙酚丁含量的测定。
In order to establish a gas chromatography-tandem mass spectrometry(GC-MS)method for the determination of dipropofol butyl in various food substrates,samples were extracted by liquid liquid with ethyl acetate,purified by PSA,separated by HP-5MS column,and determined by EI source SIM mode.317 m/z were used as quantitative ions,288,373,429 m/z as qualitative ions.Quantitative by external standard method.The linearity of dipofobutine was good in the range of 0.050~2.00 mg·L^(-1),and the correlation coefficient r2 was 0.9999.When the added concentration was 0.010,0.050 and 0.100 mg·kg^(-1)(n=6),The recoveries were 80.6%~90.5%,RSD 3.4%~4.6%,the limits of detection(S/N=3)were 0.003 mg·kg^(-1),and the limits of quantization(S/N=10)were 0.010 mg·kg^(-1).The results show that the method is stable,accurate,repeatable and sensitive,and is suitable for the determination of dipofobutyl in various foods.
作者
胡凤杨
HU Fengyang(Chinese Academy of Inspection and Quarantine Greater Bay Area,Zhongshan 528400,China)
出处
《现代食品》
2024年第24期184-188,共5页
Modern Food
关键词
气相色谱串联质谱法
食品
双丙酚丁
非法添加
gas chromatography tandem mass spectrometry
food
dipofobutyl
illegal addition
