摘要
目的探讨妊娠期高血压疾病(HDP)对子代肠道菌群结构的影响。方法选择30例HDP孕妇作为HDP组、30例正常妊娠孕妇作为对照组。收集两组孕妇子代出生后的第一次胎便进行16SrRNA测序,分析两组子代肠道菌群结构差异,以及孕妇血脂指标与子代肠道菌群的相关性。结果HDP组子代Chao1指数低于对照组(P<0.05)。在门水平上,HDP组子代肠道菌群中放线菌门、变形菌门相对丰度低于对照组,厚壁菌门、拟杆菌门的相对丰度及厚壁菌门与拟杆菌门比值高于对照组;在属水平上,HDP组子代肠道菌群中肠球菌属、葡萄球菌属、拟杆菌属、克雷伯氏菌属等致病菌属的相对丰度高于对照组,而罗氏菌属、双歧杆菌属等有益菌属的相对丰度低于对照组。从目水平到属水平,两组间共有10种细菌类群的相对丰度存在明显差异。孕妇HDL-C水平与子代肠道普雷沃氏菌属NK3B31的相对丰度呈负相关,孕妇胆固醇水平与子代肠道罗氏菌属的相对丰度呈负相关(P<0.05)。结论HDP可导致子代肠道微生态平衡被破坏,肠道菌群失调或为HDP子代发生多种疾病的重要原因之一,且孕妇血脂代谢与子代肠道菌群相关。
Objective To investigate the effect of hypertensive disorder of pregnancy(HDP)on the structure of intestinal flora in offspring.Methods A total of 30 HDP pregnant women were selected as the HDP group,and 30 normal pregnant women as the control group.The first-time meconium after the birth of offspring was collected to perform 16S rRNA sequencing.The difference of intestinal flora structure of offspring in both groups was analyzed,and the correlation of blood lipid indices of pregnant women with intestinal flora in offspring was also analyzed.Results The HDP group yielded lower Chao1 index of offspring as compared with the control group(P<0.05).At the phylum level,compared with the control group,the HDP group exhibited lower relative abundance of Actinobacteria and Proteobacteria,higher relative abundance of Firmicutes and Bacteroidetes,and a higher Firmicutes-to-Bacteroidetes ratio in intestinal flora.At the genus level,the HDP group presented higher relative abundance of pathogenic bacteria such as Enterococcus,Staphylococcus,Bacteroides,and Klebsiella in intestinal flora of offspring as compared with the control group,while the HDP group interpreted lower relative abundance of beneficial bacteria such as Roseburia and Bifidobacterium as compared with the control group.From order level to genus level,a total of 10 bacterial taxa revealed a significant difference in relative abundance between the two groups.The HDL-C level of pregnant women negatively correlated with the relative abundance of intestinal Prevotellaceae_NK3B31_group of offspring,and the cholesterol level of pregnant women negatively correlated with the relative abundance of intestinal Roseburia of offspring(P<0.05).Conclusion HDP can lead to the destruction of intestinal microecological balance in offspring,and the imbalance of intestinal flora may be one of the important reasons for the occurrence of various diseases in offspring with HDP.Maternal blood lipid metabolism is related to intestinal flora in offspring.
作者
马云云
朱珍娥
李诗雨
丁晓华
MA Yunyun;ZHU Zhene;LI Shiyu;DING Xiaohua(School of Nursing,Gansu University of Chinese Medicine,Lanzhou 730000,Gansu,China;Department of Nursing,Gansu Provincial Maternity and Child-care Hospital,Lanzhou 730050,Gansu,China)
出处
《广西医学》
2025年第1期31-37,共7页
Guangxi Medical Journal
基金
甘肃省自然科学基金(21JR11RA179)。
关键词
妊娠期高血压疾病
子代
肠道菌群
血脂
Hypertensive disorder of pregnancy
Offspring
Intestinal flora
Blood lipid
