摘要
背景近年有研究报道长链非编码RNA(lncRNA)HOTAIR、微小RNA(miRNA)-302b-3p表达异常与恶性肿瘤发生发展有关,但其其与循环肿瘤细胞关系及对预后预测价值的研究较少,值得进一步研究.目的探究LncRNA HOTAIR、miR-302b-3p表达与结肠癌循环肿瘤细胞的关系及对预后预测的价值.方法选取2019-05/2023-05临海市中医院行根治性切除术治疗的结肠癌患者160例作为观察组,另选取同期本院体检中心160例体检健康志愿者作为对照组.比较两组LncRNA HOTAIR、miR-302b-3p表达,分析LncRNA HOTAIR、miR-302b-3p表达与病理特征的关系,记录结肠癌患者术后复发转移情况,并评价LncRNA HOTAIR、miR-302b-3p表达对结肠癌术后复发转移的预测价值.结果观察组血清LncRNA HOTAIR表达高于对照组,miR-302b-3p表达低于对照组(P<0.05);多元线性回归分析显示,临床分期、淋巴结转移及循环肿瘤细胞均为结肠癌患者血清LncRNA HOTAIR、miR-302b-3p表达的影响因素(P<0.05);160例结肠癌患者失访2例,共有28例患者出现复发转移.复发转移患者血清LncRNA HOTAIR表达高于未复发转移患者,miR-302b-3p表达低于未复发转移患者(P<0.05);血清LncRNA HOTAIR、miR-302b-3p预测结肠癌患者术后复发转移风险的曲线下面积(AUC)分别为0.747(0.672-0.813)、0.785(0.713-0.846),最佳截断值分别为3.14、0.48,二者联合预测术后复发转移风险的AUC为0.880(0.919-0.926),此时敏感度与特异度分别为85.71%、75.38%,预测效能优于各指标单独预测.结论结肠癌患者血清LncRNA HOTAIR表达上调,miR-302b-3p表达下调,与临床分期、淋巴结转移、循环肿瘤细胞阳性有关,检测二者水平,有助于预测其预后.
BACKGROUND In recent years,studies have reported that the abnormal expression of the long non-coding RNA(lncRNA)HOTAIR and microRNA(miRNA)-302b-3p is associated with the occurrence and development of malignant tumors.However,there is limited research on their relationship with circulating tumor cells and their prognostic value,which warrants further investigation.AIM To investigate the relationship between the expression of HOTAIR and miR-302b-3p and circulating tumour cells in colon cancer,and to explore their value for prognostic prediction.METHODS A total of 160 patients with colon cancer who underwent radical resection at the Traditional Chinese Medicine Hospital of Linhai City from May 2019 to May 2023 were selected as an observation group,and another 160 healthy volunteers who underwent physical examination at the same hospital’s physical examination center during the same period were selected as a control group.The expression of HOTAIR and miR-302b-3p in the two groups was compared.The relationship between the expression of HOTAIR and miR-302b-3p and pathological characteristics was analyzed.The recurrence and metastasis of colon cancer patients were recorded,and the predictive value of HOTAIR and miR-302b-3p expression for the recurrence and metastasis of colon cancer was evaluated.RESULTS The expression of serum HOTAIR in the observation group was higher than that of the control group,while the expression of miR-302b-3p was lower than that of the control group(P<0.05).Multiple linear regression analysis showed that clinical stage,lymph node metastasis,and circulating tumor cells were all factors affecting the expression of serum HOTAIR and miR-302b-3p in colon cancer patients(P<0.05).Among the 160 patients with colon cancer,2 were lost to follow-up,and 28 experienced recurrence or metastasis.The expression of serum HOTAIR in patients with recurrence or metastasis was higher than that of patients without recurrence or metastasis,while the expression of miR-302b-3p was lower than that of patients without recurrence or metastasis(P<0.05).The area under the curve(AUC)of serum HOTAIR and miR-302b-3p for predicting the risk of postoperative recurrence and metastasis in patients with colon cancer was 0.747(0.672-0.813)and 0.785(0.713-0.846),respectively.The optimal cutoff values were 3.14 and 0.48,respectively.The AUC of the combination of the two for predicting the risk of postoperative recurrence and metastasis was 0.880(0.919-0.926),with a sensitivity and specificity of 85.71%and 75.38%,respectively.The predictive efficacy was superior to that of each indicator alone.CONCLUSION The expression of serum HOTAIR is up-regulated and the expression of miR-302b-3p is down-regulated in patients with colon cancer,which is related to clinical stage,lymph node metastasis,and positive circulating tumor cells.Detecting the levels of both can help predict patient prognosis.
作者
王凌
林霞
戚国勇
陈贵平
Ling Wang;Xia Lin;Guo-Yong Qi;Gui-Ping Chen(Department of Gastroenterology,Linhai Hospital of Traditional Chinese Medicine,Taizhou 317000,Zhejiang Province,China;Department of Pathology,Linhai First People’s Hospital,Taizhou 317000,Zhejiang Province,China;Department of Gastrointestinal Surgery,Zhejiang Provincial Hospital of Traditional Chinese Medicine,Hangzhou 310001,Zhejiang Province,China)
出处
《世界华人消化杂志》
2025年第2期140-147,共8页
World Chinese Journal of Digestology
