摘要
目的分析不同性状特征的何首乌饮片化学成分组织蓄积差异,明确性状特征、化学成分及组织间的差异及相关性。方法采用超高效液相色谱-三重四极杆质谱法(UPLC-QQQ-MS/MS)测定何首乌饮片不同组织中反式-2,3,5,4’-四羟基二苯乙-2-O-β-D-葡萄糖苷、大黄素、大黄素-8-O-β-D-葡萄糖苷、大黄素甲醚和大黄素甲醚-8-O-β-D-葡萄糖苷5个化学成分的含量,采用单因素方差分析(ANOVA)、Pearson相关性分析等统计方法比较化学成分在不同性状特征的何首乌4个组织分布特点,及其与不同组织的相关性。结果反式-2,3,5,4’-四羟基二苯乙-2-O-β-D-葡萄糖苷均明显蓄积在栓皮中;单因素方差分析结果显示,5个化学成分在不同性状特征的何首乌中组织分布均存在显著差异;相关性分析结果显示,广东高州、广东德庆和云南曲靖产地的何首乌中5个化学成分含量与部位均有显著相关(P<0.05);云南曲靖产地的何首乌中央和云锦的大黄素-8-O-β-D-葡萄糖苷呈正相关(P<0.05);广东高州产地的何首乌栓皮与云锦的大黄素-8-O-β-D-葡萄糖苷呈负相关(P<0.05),中央与云锦的二苯乙烯苷呈正相关(P<0.05)。结论明确了化学成分在不同性状特征何首乌的组织中的分布规律,为何首乌质量评价提供参考。
Objective To analyze the differences in tissue accumulation of chemical components in Polygonum multiflorum Thunb.pieces with different characters and characteristics,and to reveal the differences and correlations between characters,chemical components and tissues.Methods The contents of stilbene glucoside,emodin,emodin-8-O-β-Dglucoside,emodin methyl ether and emodin methyl ether-8-O-β-D-glucoside in different tissues of Polygonum multiflorum Thunb were determined with UPLC-QQQ-MS/MS,and the distribution of chemical components in four tissues of Polygonum multiflorum Thunb.with different characteristics was compared while their correlations with different tissues were explored using such statistical methods as ANOVA and Pearson correlation analysis.Results Trans-2,3,5,4’-tetrahydroxydiphenyl ethyl-2-O-β-D-glucoside was obviously accumulated in the cork.The results of one-way ANOVA showed that there were significant differences in the tissue distribution of five main chemical components in Polygonum multiflorum Thunb.The results of correlation analysis showed that the contents of five chemical components in Polygonum multiflorum Thunb.from Gaozhou and Deqing,Guangdong and Qujing,Yunnan were significantly correlated with their parts(P<0.05).There was a positive correlation between the center of Polygonum multiflorum Thunb.from Qujing,Yunnan and emodin-8-O-β-D-glucoside from Yunjin(P<0.05).The cork of Polygonum multiflorum Thunb.from Gaozhou,Guangdong was negatively correlated with emodin-8-O-β-D-glucoside of abnormal vascular bundle(P<0.05),and the central vascular bundles of Polygonum multiflorum Thunb.was positively correlated with stilbene glucoside of abnormal vascular bundle of Polygonum multiflorum Thunb.(P<0.05).The results of one-way ANOVA showed that there were significant differences in the tissue distribution of five main chemical components in Polygonum multiflorum Thunb.The results of correlation analysis showed that the contents of five chemical components in Polygonum multiflorum Thunb.from Gaozhou and Deqing,Guangdong,Qujing Yunnan were significantly correlated with their parts(P<0.05).There was a positive correlation between the central vascular bundles of Polygonum multiflorum Thunb.from Qujing,Yunnan and emodin-8-O-β-D-glucoside from abnormal vascular bundle(P<0.05).The cork of Polygonum multiflorum Thunb.from Gaozhou,Guangdong was negatively correlated with emodin-8-O-β-D-glucoside of Yunjin(P<0.05),and the central vascular bundles of Polygonum multiflorum Thunb.was positively correlated with stilbene glucoside of abnormal vascular bundle(P<0.05).Conclusion The distribution of chemical components in Polygonum multiflorum Thunb.tissues with different characters and characteristics has been characterized,which provides data for quality evaluation of Polygonum multiflorum Thunb.
作者
汪祺
杨颜榕
李妍怡
谢晶鑫
魏锋
马双成
王芳
石佳
WANG Qi;YANG Yanrong;LI Yanyi;XIE Jingxin;WEI Feng;MA Shuangcheng;WANG Fang;SHI Jia(National Institutes for Food and Drug Control,Institute for Control of Chinese Traditional Medicine and Ethnic Medicine,Beijing 100050,China;Shenyang Pharmaceutical University,School of Functional Food and Wine,Shenyang Liaoning 110016,China;School of Chinese Materia Medica,Beijing University of Chinese Medicine,Beijing 102400,China;National Institutes for Food and Drug Control,Standard Materials and Standardization Management Center,Beijing 100050,China;Chinese Pharmacopoeia Commission,Beijing 100061,China;State Key Laboratory of Drug Regulatory Science,Beijing 100061,China)
出处
《中国药物警戒》
2024年第12期1371-1376,共6页
Chinese Journal of Pharmacovigilance
基金
国家自然科学基金资助项目(82374033)。
关键词
何首乌
性状
二苯乙烯苷类成分
蒽醌类成分
组织分布
相关性
Polygonum multiflorum Thunb.
Character
Stilbene Glycosides
Anthraquinone
Distribution
Correlation
