摘要
研究旨在探讨环二肽Cyclo(Leu-Pro)对植物乳杆菌生长的影响。该研究以植物乳杆菌(Lactobacillus Plantarum T1)为研究对象,采用高效液相色谱法初步定性定量植物乳杆菌T1发酵液中的Cyclo(Leu-Pro),然后将植物乳杆菌T1分别接种于不含或含不同质量浓度(0.5、1、5、10μg/mL)Cyclo(Leu-Pro)的MRS肉汤培养基中培养18 h,采用96孔法和平板计数法测定不同培养条件下植物乳杆菌T1的生长曲线和活菌数。结果表明:不同质量浓度的Cyclo(Leu-Pro)对植物乳杆菌T1的生长均有明显的促生长作用,其中,1μg/mL Cyclo(Leu-Pro)的促生长效果最佳,可将活菌数提高到8.73×10^(10) CFU/mL,相比于对照组提高了一个数量级。在不同胁迫条件下,1μg/mL Cyclo(Leu-Pro)能使植物乳杆菌T1的延滞期缩短、生长速度提高,其活菌数和生物量显著增加(P<0.05)。综上所述,Cyclo(Leu-Pro)促进了植物乳杆菌T1的生长,增强了其抗胁迫能力。这将有利于植物乳杆菌T1作为发酵剂在食品发酵领域的应用。
In this study,it’s the effect of Cycle(Leu Pro)on the growth of Lactobacillus plantarum was investigated.Lactobacillus Plantarum T1 was used as the research object,and high-performance liquid chromatography(HPLC)was used to preliminarily characterize and quantify the Cyclo(Leu-Pro)in the fermentation broth of Lactobacillus Plantarum T1.Lactobacillus Plantarum T1 was respectively inoculated in the MRS broth medium without or with different mass concentrations(0.5,1,5,10μg/mL)of Cyclo(Leu-Pro)for 18 h.The growth curves and viable bacterial counts of Lactobacillus plantarum T1 under different culture conditions were determined by the 96-well method and plate counting method.The results showed that Cyclo(Leu-Pro)at different mass concentrations had significant growth-promoting effects on the growth of Lactobacillus plantarum T1,among which,1μg/mL Cyclo(Leu-Pro)had the greatest growth-promoting effect and could increase the viable counts to 8.73×10^(10) CFU/mL,which was an order of magnitude higher than the control.Under different stress conditions,1μg/mL Cyclo(Leu-Pro)could shorten the lag period and increase the growth rate of Lactobacillus plantarum T1,and its viable count and biomass increased significantly(P<0.05).In summary,Cyclo(Leu-Pro)promoted the growth of Lactobacillus plantarum T1 and enhanced its stress resistance.This will favor the application of Lactobacillus plantarum T1 as a starter in the field of food fermentation.
作者
谭旭
吴忠坤
夏迪
高宁
王丽
高瑶
郭新梅
刘学聪
王远亮
TAN Xu;WU Zhongkun;XIA Di;GAO Ning;WANG Li;GAO Yao;GUO Xinmei;LIU Xuecong;WANG Yuanliang(College of Food Science and Technology,Hunan Agricultural University,Changsha 410128,China;Ausnutria Dairy(China)Limited,Changsha 410005,China;Jinqiao Biotechnology Company Limited,Huai'an 223200,China)
出处
《现代食品科技》
北大核心
2025年第1期81-88,共8页
Modern Food Science and Technology
基金
国家自然科学基金项目(31701606)
淮安市重点实验项目(HA202310)。
