摘要
目的建立4–巯基苯甲酸/多肽修饰金纳米粒子修饰的表面增强拉曼光谱(surface enhanced Raman spectroscopy,SERS)传感器快速检测水中的Ag^(+)。方法通过特异性的Ag^(+)多肽识别序列(RFPRGGDD)和4–巯基苯甲酸的结合,构建了Ag^(+)表面增强拉曼光谱传感器,使用便携式拉曼光谱仪读取数据。结果在10~850 nmol/L的浓度范围内线性关系较好,相关系数(r^(2))为0.98345,检出限为36 pmol/L,加标回收率为95.28%~102.9%,相对标准偏差在0.79%~3.73%。结论该方法响应速度快、选择性好、检出限低,适用于现场快速检测水中的Ag^(+)。
Objective A 4-mercaptobenzoic acid/polypeptide-modified gold nanoparticle modified surface enhanced Raman spectroscopy(SERS)sensor was developed for rapid detection of Ag^(+)in water.Methods A Ag^(+)surface enhanced Raman spectroscopy sensor was constructed by combining a specific Ag^(+)peptide recognition sequence(RFPRGGDD)with 4-mercaptobenzoic acid,and data was read using a portable Raman spectrometer.Results In the concentration range of 10~850 nmol/L,the linear relationship was good,the correlation coefficient(r^(2))was 0.98345,the limit of detection was 36 pmol/L,the standard recoveries were 95.28%~102.9%,and the relative standard deviations were 0.79%~3.73%.Conclusion The method has high response speed,good selectivity and low detection limit,and is suitable for rapid detection of Ag^(+)in water.
作者
容培森
李皓
高汉铭
许庆鹏
RONG Pei-Sen;LI Hao;GAO Han-Ming;XU Qing-Peng(Guangdong Testing Institute of Product Quality Supervision,Foshan 528300,China)
出处
《实验室检测》
2024年第9期9-13,共5页
Laboratory Testing
