摘要
目的对荧光定量逆转录聚合酶链式反应(RT-PCR)法在病原微生物检验中的应用结果进行分析。方法回顾性分析我院检验科于2023年1月14日—2023年12月14日期间检验的7502份微生物检验资料,分别实施了常规RT-PCR法及荧光定量RT-PCR法检验,金标准为特殊染色镜检结果,分析两种检验结果的诊断效能等指标差异。结果(1)金标准检测出阴性、阳性占比为490∶7012,常规RT-PCR法检验结果为阴性、阳性为:479∶6358,荧光定量RT-PCR法检验阴性、阳性比为:488∶7010。(2)荧光定量RT-PCR法的灵敏度为99.97%、特异度为99.59%、准确度为99.95%,均高于常规RT-PCR法,有统计学意义(P<0.05)。(3)荧光定量RT-PCR法检验各类微生物检出率依次为99.91%、100.00%、100.00%、99.91%、100.00%、100.00%、99.97%,高于常规RT-PCR法,有统计学意义(P<0.05)。结论荧光定量RT-PCR法在病原微生物检验中诊断效能及各类微生物检出率高,能够为医生诊疗提供科学依据,可推行。
Objective To analyze the application results of fluorescence quantitative reverse transcription-polymerase chain reaction(RT-PCR)in the detection of pathogenic microorganisms.Methods A retrospective analysis was made of 7502 microbiological test data examined in the laboratory of our hospital from January 14th,2023 to December 14th,2023.Conventional RT-PCR method and fluorescent quantitative RT-PCR method were performed respectively,and the gold standard was the results of special staining microscopy.The diagnostic efficiency and other indicators of the two test results were analyzed.Results①The ratio of negative to positive by gold standard was 490∶7012,the ratio of negative to positive by conventional RT-PCR was 479∶6358,and the ratio of negative to positive by fluorescence quantitative RTPCR was 488∶7010.②The sensitivity,specificity and accuracy of fluorescence quantitative RT-PCR method were 99.97%,99.59%and 99.95%,which were higher than that of conventional RT-PCR method,with statisticalsignificance(P<0.05).③The detection rates of various microorganisms by fluorescence quantitative RT-PCR were 99.91%,100.00%,100.00%,99.91%,100.00%,100.00%,99.97%,higher than that by conventional RT-PCR,with statistical significance(P<0.05).Conclusion Fluorescence quantitative RT-PCR method has high diagnostic efficiency and detection rate in pathogenic microorganisms,which can provide scientific basis for doctors’diagnosis and treatment and can be implemented.
作者
郭金芳
GUO Jin-Fang(Department of Clinical Laboratory,The Second People’s Hospital of Binzhou,Binzhou 256800,China)
出处
《实验室检测》
2024年第10期162-164,共3页
Laboratory Testing
关键词
荧光定量逆转录聚合酶链式反应法
病原微生物
检验
常规逆转录聚合酶链式反应法
支原体
fluorescence quantitative reverse transcription-polymerase chain reaction method
pathogenic microorganism
inspect
conventional reverse transcription-polymerase chain reaction method
mycoplasma
