摘要
目的 调查海南省部分市县市售水果中大肠埃希菌(Escherichia coli)的污染分布情况,并对分离菌株进行耐药性分析,旨在识别食源性大肠埃希菌的风险并评估其对食品安全的潜在影响。方法 对样品中的大肠埃希菌进行分离鉴定,采用VITEK 2法检测大肠埃希菌分离株的耐药性。结果 在240份水果样品中共分离出大肠埃希菌51株,检出率为21.3%,其中19株大肠埃希菌具有耐药性,耐药率为37.3%,包括7株单重耐药和12株菌多重耐药。通过比较分析不同地区和不同水果中大肠埃希菌的分布情况,发现水果种类和采样地点对大肠埃希菌的分布有显著影响。结论 在市售水果中大肠埃希菌的分布比较广泛,且存在耐药情况,应加强对水果中病原菌分布和耐药性的检测,同时这项研究也为食品安全管理和监测提供了重要参考依据。
Objective To investigate the distribution of Escherichia coli(E.coli)contamination in fruits sold in some cities and counties of Hainan Province,and to analyze the isolates for drug resistance,aiming at identifying the risk of foodborne E.coli and evaluating its potential impact on food safety.Methods Isolation and identification of E.coli in samples and detection of drug resistance of E.coli isolates by VITEK 2 method.Results A total of 51 strains of E.coli were isolated from 240 fruit samples,with a detection rate of 21.3%.19 strains of E.coli were drug-resistant,with a resistance rate of 37.3%,including 7 single-resistant and 12 multi-resistant strains.Comparative analysis of the distribution of E.coli in different regions and fruits showed that fruit species and sampling locations had a significant effect on the distribution of Escherichia coli.Conclusion The distribution of E.coli in commercially available fruits is widespread and drug-resistant,the detection of pathogenic bacteria distribution and drug resistance in fruits should be strengthened,and this study also provides an important reference basis for food safety management and monitoring.
作者
袁维道
冯春艳
陈彩霞
王雪吟
冯晨韵
YUAN Wei-Dao;FENG Chun-Yan;CHEN Cai-Xia;WANG Xue-Yin;FENG Chen-Yun(Hainan Academy of Inspection and Testing,Haikou 570203,China)
出处
《实验室检测》
2024年第8期125-128,共4页
Laboratory Testing
关键词
大肠埃希菌
耐药性
分布特征
水果
Escherichia coli
drug resistance
distribution characteristics
fruits
