摘要
目的 优化喜马拉雅紫茉莉中黄酮、多酚、皂苷的超声提取工艺,并多体系综合评价其抗氧化活性。方法 在乙醇浓度、液料比、提取温度、提取时间的单因素实验的基础上,以综合评分值作为评价指标,采用BoxBehnken响应面法对黄酮、多酚、皂苷进行提取工艺优化,并通过对DPPH、ABTS、超氧阴离子、羟基自由基的清除率对其体外抗氧化性进行考察。结果 优化得到的超声提取工艺为乙醇浓度60%、液料比30 mL/g、提取温度50℃、提取时间55 min。提取物对各自由基均具有较强的清除作用。结论 经验证,优化得到的喜马拉雅紫茉莉中黄酮、多酚、皂苷化合物的超声提取工艺合理,提取物具有体外抗氧化的活性。
Objective To optimize the ultrasonic extraction technology of flavonoids,polyphenols and saponins from Mirabilis himalaica,and to evaluate their antioxidant activities.Methods Based on the single factor tests of ethanol concentration,liquid-solid ratio,extraction temperature and extraction time,Box-Behnken response surface method was used to optimize the extraction process of flavonoids,polyphenols and saponins.The antioxidant activity of DPPH,ABTS,superoxide anion and hydroxyl radical was investigated in vitro.Results The optimized ultrasonic extraction technology was 60%ethanol concentration,30 mL/g liquid-solid ratio,50℃extraction temperature and 55 min extraction time.The extracts had strong scavenging effect on free radicals.Conclusion It is proved that the optimized ultrasonic extraction technology of flavonoids,polyphenols and saponins from Mirabilis himalaica is reasonable,and the extracts have antioxidant activity in vitro.
作者
王月玲
扎西卓玛
王永珍
戚鹏飞
徐雪梅
杨金草
WANG Yue-Ling;ZHAXI Zhuo-Ma;WANG Yong-Zhen;QI Peng-Fei;XU Xue-Mei;YANG Jin-Cao(Lanzhou Institute of Food and Drug Inspection and Testing,Lanzhou 730050,China;Gannan Tibetan Autonomous Prefecture Drug Inspection and Testing Center,Hezuo 747000,China;147th Hospital of the 8th Division of Xinjiang Production and Construction Corps,Shihezi 832045,China)
出处
《实验室检测》
2024年第8期156-160,共5页
Laboratory Testing
基金
甘肃省药品监督管理局药品科研项目(2021GSMPA006)
兰州市科学技术局兰州市科技计划项目(2023-ZD-232)
关键词
喜马拉雅紫茉莉
超声提取工艺
体外抗氧化性
Mirabilis himalaica
ultrasonic extraction technology
in vitro antioxidant activity
