摘要
本文旨在研究4-萜品醇对过氧化氢诱导的氧化应激断奶仔猪肺脏、脾脏和胰腺抗氧化功能的调节作用。试验选取32头28日龄的健康“杜×长×大”断奶仔猪,平均体重(8.53±0.42)kg,随机分为4个组,每组8头猪。对照组和过氧化氢组仔猪饲喂基础饲粮,4-萜品醇组和过氧化氢+4-萜品醇组仔猪饲喂基础饲粮+60 mg/kg 4-萜品醇。预试期3 d,正试期21 d。正试期第15天和第18天,过氧化氢组和过氧化氢+4-萜品醇组分别腹腔注射1 mL/kg BW的10%过氧化氢,对照组和4-萜品醇组分别腹腔注射等量的0.9%无菌生理盐水。结果表明:1)与对照组相比,过氧化氢组血清丙二醛(MDA)含量显著提高(P<0.05),总抗氧化能力(T-AOC)和过氧化氢酶(CAT)活性显著降低(P<0.05);与过氧化氢组相比,过氧化氢+4-萜品醇组血清MDA含量极显著降低(P<0.01),超氧化物歧化酶(SOD)活性和T-AOC显著提高(P<0.05),CAT活性极显著提高(P<0.01)。2)与对照组相比,过氧化氢组肺脏MDA含量极显著提高(P<0.01),CAT活性显著降低(P<0.05),T-AOC极显著降低(P<0.01);与过氧化氢组相比,过氧化氢+4-萜品醇组肺脏MDA含量极显著降低(P<0.01),CAT活性极显著提高(P<0.01)。3)与对照组相比,过氧化氢组脾脏MDA含量极显著提高(P<0.01),CAT活性显著降低(P<0.05),T-AOC极显著降低(P<0.01);与过氧化氢组相比,过氧化氢+4-萜品醇组脾脏MDA含量极显著降低(P<0.01),CAT活性极显著提高(P<0.01)。4)与对照组相比,过氧化氢组胰腺MDA含量极显著提高(P<0.01),SOD活性显著降低(P<0.05),CAT活性和T-AOC极显著降低(P<0.01);与过氧化氢组相比,过氧化氢+4-萜品醇组胰腺MDA含量显著降低(P<0.05),CAT活性极显著提高(P<0.01)。综上所述,饲粮中添加4-萜品醇可以缓解过氧化氢诱导的氧化损伤,进而缓解断奶仔猪内脏器官的氧化应激。
This experiment aimed to investigate the regulation of terpinen-4-ol on antioxidant function of lungs,spleen and pancreas of weaned piglets with oxidative stress induced by hydrogen peroxide.Thirty-two healthy 28-day-old“Duroc×Landrace×Large White”weaned piglets with similar body weight of(8.530.42)kg were randomly divided four groups with 8 pigs per group.Piglets in the control and hydrogen peroxide groups were fed a basal diet,while others in the terpinen-4-ol and hydrogen peroxide+terpinen-4-ol groups were fed the basal feed+60 mg/kg terpinen-4-ol.The pre-experimental period lasted for 3 days,and the experimental period lasted for 21 days.On the 15th and 18th days of the experimental period,the hydrogen peroxide and hydrogen peroxide+terpinen-4-ol groups were injected with 1 mL/kg BW 10%hydrogen peroxide intraperitoneally,while the control and terpinen-4-ol groups were injected with equivalent 0.9%sterile physiological saline intraperitoneally.The results showed as follows:1)compared with the control group,the serum malondialdehyde(MDA)content of the hydrogen peroxide group was significantly increased(P<0.05),and the total antioxidant capacity(T-AOC)and catalase(CAT)activity were significantly decreased(P<0.05);compared with the hydrogen peroxide group,the serum MDA content of the hydrogen peroxide+terpinen-4-ol group was significantly decreased(P<0.01),the superoxide dismutase(SOD)activity and T-AOC were significantly increased(P<0.05),and the CAT activity was significantly increased(P<0.01).2)Compared with the control group,the lungs MDA content of hydrogen peroxide group was significantly increased(P<0.01),the CAT activity was significantly decreased(P<0.05),and the T-AOC was significantly decreased(P<0.01);compared with the hydrogen peroxide group,the lungs MDA content of the hydrogen peroxide+terpinen-4-ol group was significantly decreased(P<0.01),and the CAT activity was significantly increased(P<0.01).3)Compared with the control group,the spleen MDA content of the hydrogen peroxide group was significantly increased(P<0.01),the CAT activity was significantly decreased(P<0.05),and the T-AOC was significantly decreased(P<0.01);compared with the hydrogen peroxide group,the spleen MDA content of hydrogen peroxide+terpinen-4-ol group was significantly decreased(P<0.01),and the CAT activity was significantly increased(P<0.01).4)Compared with the control group,the pancreas MDA content of the hydrogen peroxide group was significantly increased(P<0.01),the SOD activity was significantly decreased(P<0.05),and the CAT activity and T-AOC were significantly decreased(P<0.01);compared with the hydrogen peroxide group,the pancreas MDA content of the hydrogen peroxide+terpinen-4-ol group was significantly decreased(P<0.05),and the CAT activity was significantly increased(P<0.01).In conclusion,the addition of terpinen-4-ol in the diet can alleviate the oxidative damage induced by hydrogen peroxide stimulation,and then alleviate the oxidative stress in internal organs of weaned piglets.
作者
周杨淑
高畅
宋丽
董丽
喻礼怀
ZHOU Yangshu;GAO Chang;SONG Li;DONG Li;YU Lihuai(College of Animal Science and Technology,Yangzhou University,Yangzhou 225009,China)
出处
《动物营养学报》
北大核心
2025年第1期226-235,共10页
CHINESE JOURNAL OF ANIMAL NUTRITION
基金
国家自然科学青年基金(32302753)
基于类器官模型研究Me6对仔猪肠上皮细胞稳态的调节作用及其应用(SJCX23_1992)。
关键词
4-萜品醇
氧化应激
断奶仔猪
肺脏
胰腺
脾脏
抗氧化
terpinen-4-ol
oxidative stress
weaned piglets
lung
spleen
pancreas
antioxidant
