摘要
目的建立高效液相色谱法精确定量保健食品中烟酰胺单核苷酸(nicotinamide mononucleotide,NMN)与烟酰胺腺嘌呤双核苷酸(nicotinamide adenine dinucleotide,NAD+)的含量的分析方法。方法采用Waters BEH-Amide色谱柱,0.1%磷酸溶液和乙腈为流动相,进行梯度洗脱,流速设定为1.0 m L/min,进样量为2μL,柱温箱温度设置为35℃,选择263 nm作为检测波长。保健食品用60%甲醇溶液超声提取10 min,将提取液通过微孔滤膜过滤后进行高效液相色谱法测定,外标法定量。结果在质量浓度10~1000μg/m L的范围内,NMN和NAD^(+)均展现出优异的线性关系,其线性相关系数(r)均不低于0.999,该方法的定量限为0.50 g/kg,NMN和NAD+的回收率在95.9%~98.8%范围内,相对标准偏差在1.19%~3.05%之间。结论该方法可以准确测定保健食品中NMN和NAD+含量。
Objective To establish an analytical mehod for the determination of nicotinamide mononucleotide(NMN)and nicotinamide adenine dinucleotide(NAD+)in health food by high performance liquid chromatography.Methods Determination was performed on Waters BEH-Amide column with mobile phase consisting of 0.1%phosphoric acid solution-acetonitrile(gradient elution).The flow rate was 1.0 mL/min,and the sample size was 2μL.The column temperature was 35℃,and the detection wavelength was set at 263 nm.Health food was extracted with 60%methanol solution in ultrasonic water bath for 10 min.The extract was filtered through micoron filter,and analyzed by high performance liquid chromatography.Results The results showed that NMN and NAD^(+)exhibited excellent linear relationships,the correlation coefficient(r)was over 0.999 in the range of 10–1000μg/mL.The method limit of quantitation was 0.50 g/kg.Recoveries were ranged from 95.9%to 98.8%,and relative standard deviations were ranged from 1.19%to 3.05%.Conclusion This method is accurate and suitable to determine NMN and NAD+in health food.
作者
解楠
郑国建
XIE Nan;ZHENG Guo-Jian(Shanghai Institute of Quality Inspection and Technical Research,Shanghai 200233,China)
出处
《食品安全质量检测学报》
2025年第2期224-230,共7页
Journal of Food Safety and Quality
关键词
烟酰胺单核苷酸
烟酰胺腺嘌呤双核苷酸
保健食品
高效液相色谱法
nicotinamide mononucleotide
nicotinamide adenine dinucleotide
health food
high performance liquid chromatography
