摘要
建立高效液相色谱法同时测定骨痹灵片中葛根素、阿魏酸、橙皮苷、蛇床子素和异欧前胡素。采用CAPCELL PAK C_(18)色谱柱(250 mm×4.6 mm,5μm),以乙腈-0.1%甲酸溶液为流动相进行梯度洗脱,流量为1.0 mL/min,柱温为30℃,检测波长为250 nm(葛根素)、284 nm(橙皮苷)和322 nm(阿魏酸、蛇床子素、异欧前胡素),进样体积为10μL。5种成分在各自质量浓度范围内与对应的色谱峰面积线性关系良好,相关系数均为0.9999,方法检出限为0.04~0.14μg/mL,定量限为0.13~0.46μg/mL。样品加标平均回收率为96.0%~103.1%,测定结果的相对标准偏差为0.8~1.7%(n=6)。该方法简便可靠,重复性好,可用于骨痹灵片的质量控制。
A method for simultaneous determination of puerarin,ferulic acid,hesperidin,osthole and isoimperatorin in Gubiling Tablets by high performance liquid chromatography was established.CAPCELL PAK C_(18)column(250 mm×4.6 mm,5μm)was used for gradient elution with acetonitrile-0.1%formic acid solution as the mobile phase at a flow rate of 1.0 mL/min.The column temperature was 30℃.The detection wavelengths were set at 250 nm for puerarin,284 nm for hesperidin,322 nm for ferulic acid,osthole and isoimperatorin,respectively.The injection volume was 10μL.The mass concentrations of the 5 constituents had a good linear relationships with the corresponding chromatographic peak areas in their respective ranges,and the correlation coefficients were all 0.9999.The detection limits were 0.04-0.14μg/mL,and the quantification limits were 0.13-0.46μg/mL.The average recoveries of sample spiked were 96.0%-103.1%.and the relative standard deviations of the determination results were 0.8%-1.7%(n=6).This method is simple,reliable,and has good reproducibility,which can be used for quality control of Gubiling Tablets.
作者
丁爱华
朱干红
曹桂萍
陈玲玲
滕习羽
支荣荣
周会芹
DING Aihua;ZHU Ganhong;CAO Guiping;CHEN Lingling;TENG Xiyu;ZHI Rongrong;ZHOU Huiqin(Yancheng Food and Drug Supervision and Testing Center,Yancheng 224055,China;Department of Pharmacy,Yancheng TCM Hospital Affiliated to Nanjing University of Chinese Medicine,Yancheng 224001,China;Hanlin College,Nanjing University of Chinese Medicine,Taizhou 225300,China)
出处
《化学分析计量》
2025年第1期100-105,共6页
Chemical Analysis And Meterage
关键词
骨痹灵片
高效液相色谱法
葛根素
阿魏酸
橙皮苷
蛇床子素
异欧前胡素
Gubiling Tablets
high performance liquid chromatography
puerarin
ferulic acid
hesperidin
osthole
isoimperatorin
