摘要
采用傅立叶变换红外光谱仪采集了不同温度、不同部位、不同批次洋甘菊药材的红外光谱及二阶导数红外光谱,并结合双指标序列分析法、聚类分析法对不同批次洋甘菊药材进行了全面评价。结果表明,从洋甘菊红外光谱中确定了19个共有峰,根据不同温度下各特征吸收峰的变化,推测温度可能影响洋甘菊中黄酮类、酯类、糖(苷)类物质;洋甘菊花、茎的红外光谱具有一定的相似性和差异性,根据特征吸收峰强度推测花中黄酮类、酚酸类含量较高;不同批次洋甘菊的峰位、峰强度存在一定相似性和差异性,二阶导数红外光谱可以分离出红外光谱中一些相互重叠的吸收峰;不同批次洋甘菊样品间共有峰率为60.00%~100.00%,变异峰率为0.00~47.62%;当欧氏距离为5~10时,16批次洋甘菊药材可聚为3类,其中S1、S4、S5、S6、S7、S10、S12聚为一类,S3、S8、S9、S11、S13、S15、S16聚为一类,S2、S14聚为一类。该方法通过不同角度对洋甘菊药材的差异进行分析评价,具有一定的可行性,为药材的质量控制提供了新方法。
We collected infrared spectra and second derivative infrared spectra of Matricaria chamomilla L.from different temperatures,different parts,and different batches by Fourier transform infrared spectroscopy,and evaluated different batches of Matricaria chamomilla L.by infrared spectra and second derivative infrared spectra combined with dual-index sequence analysis and cluster analysis.The results show that 19 common peaks are identified from the infrared spectra of Matricaria chamomilla L.,and the characteristic peaks of samples vary under different temperatures,indicating that temperature may affect flavonoids,esters,and glyco-sides from Matricaria chamomilla L..The infrared spectra of flowers and stems of Matricaria chamomilla L.have certain similarities and differences.According to the intensity of characteristic absorption peak,it is specu-lated that the contents of flavonoids and phenolic acids in flowers are higher.The peaks and intensities of differ-ent batches of Matricaria chamomilla L.have certain similarities and differences.The second derivative infra-red spectra can separate some overlapping absorption peaks in the infrared spectra.The common peak rates of different batches of Matricaria chamomilla L.samples are 60.00%-100.00%,and the variant peak rates are 0.00-47.62%.When the Euclidean distance is 5-10,sixteen batches of Matricaria chamomilla L.can be clustered into 3 categories,among which S1,S4,S5,S6,S7,S10,and S12 are clustered into one category;S3,S8,S9,S11,S13,S15,and S16 are grouped into one category;S2 and S14 are categorized into one category.This method analyzes and evaluates the differences of Matricaria chamomilla L.herbs from different angles,which has certain feasibility and provides a new method for the quality control of medicinal herbs.
作者
杨青青
刘婷
张明惠
马璇
姚军
YANG Qingqing;LIU Ting;ZHANG Minghui;MA Xuan;YAO Jun(New Cicon Pharmaceutical Co.,Ltd.,Urumqi 830011,China;Xinjiang Qimu Pharmaceutical Research Institute,Urumqi 830011,China;College of Pharmacy,Xinjiang Medical University,Urumqi 830054,China;Xinjiang Key Laboratory of Generic Technology of Traditional Chinese Medicine(Ethnic Medicine)Pharmacy,Urumqi 830011,China)
出处
《化学与生物工程》
北大核心
2025年第1期62-68,共7页
Chemistry & Bioengineering
基金
新疆优势药用植物管花肉苁蓉和甘草的化学药关键技术研究(2022A03016-1)。
关键词
洋甘菊
红外光谱
双指标序列分析法
聚类分析法
Matricaria chamomilla L.
infrared spectrum
dual-index sequence analysis
cluster analysis
