摘要
目的探讨低剂量环磷酰胺对烧伤大鼠全身炎症介质综合征 (SIRS)的控制作用。方法以大鼠为实验动物 ,分为空白组、对照组、实验组。对照组、实验组制作 5 0 %Ⅲ°烫伤模型 ,实验组于伤后 1小时腹腔内注射低剂量环磷酰胺 (2mg/kg) ,对照组腹腔内注射等量生理盐水。分别在伤后 2h、3h、6h测量 2组血液及肝脏组织中肿瘤坏死因子 (TNF -a)的含量。喂养至动物死亡观察两组动物的生存时间。结果实验组、对照组伤后 2小时血液及肝脏内TNF -a的含量明显高于未烧伤的空白组 (P <0 0 5 )。实验组伤后 2h、3h、6h血液及肝脏内TNF -a的含量明显低于对照组 (P <0 0 5 )。实验组动物的生存时间明显延长 (P <0 0 5 )。结论低剂量环磷酰胺能够减少烧伤后炎症介质的释放 ,能减轻烧伤后SIRS的发生。
Objective To study the effect of low dose cyclophosphamide on SIRS (systemic inflammatory response syndrome)in early stage of burns.Methods The mice were divided into three groups,the blank group,control group and experimental group.The burned model were found in control group and experimental group.After one hour burn,cyclophosphamide was used in experimental group and physiological saline was used in control group.After 2h,3h,6h burn,TNF-a was tested in experimental group and control group.At the same time,the survival time was observed in the two groups.Results Comparing with the blank group,TNF-a obvious increasing in experimental group and control group ( P <0.05).After 2h,3h and 6h burn,the TNF-a in experimental group was lower obviously than that in control group( P <0.05),and the survival time in experimental group was longer obviously than that in control group( P <0.05) Conclusion The low dose cyclophosphamide can reduce inflammatory transmitter releasing and lessen SIRS in early stage of burn.
出处
《贵州医药》
CAS
2002年第12期1080-1082,共3页
Guizhou Medical Journal
基金
贵州省卫生厅青年基金项目
