摘要
为研究悬浮红细胞 (CRCs)在体外 4℃保存过程中血小板 白细胞聚合物 (PLA)形成的数量及其变化特点及使用滤器的预防效果 ,将每袋 8个单位分离血浆或富含血小板血浆的CRCs等分为两袋 ,即对照组和滤器过滤组。当天和每周测定对照组和过滤组CRCs内血小板、白细胞计数和血小板 淋巴细胞 (P Lym)聚合物、血小板 单核细胞 (P Mon)聚合物、血小板 中性粒细胞 (P Neu)聚合物及总的PLA百分比率及浓度。结果显示 ,两组经过 4℃保存后 ,CRCs所含PLA比率和浓度都显著升高。与体内循环血液比较 ,P lym、P mon升高 2～ 5倍 ,P neu升高 10～ 2 0倍 ,其中分离血浆制备的CRCs在第 1天就已经显著升高。CRCs内PLA在保存 1周时浓度和比率最高 ,然后缓慢下降 ,但始终保持较高的水平。P neu占总PLA的 80 %～ 90 %。过滤组在保存过程中 ,利用流式细胞术未能检测出其产生的PLA。研究发现 ,在 4℃保存CRCs内检测出高浓度的PLA ,利用去白细胞滤器能有效防止PLA的产生。
To study the formation and change of platelet leucocyte aggregation (PLA) in red blood cell concentrations(CRCs) stored at 4℃ and the preventive effectiveness of filtration, 8 units of CRCs were each divided into two equal parts in two integrative bags and two paired groups were formed: control group and filtration group. The following items of control group and filtration group were detected weekly. Platelet and leukocyte counts, concentration and percentage of platelet lympocyte (P lym), platelet monocyte (P mon), platelet neutrophil (P neu) aggregatoins, and concentration of total PLA. The results showed that rate and concentration of PLA in the control group were significanly higher than those in the circulating blood. The rates of P lym and P mon in CRCs stored at 4 ℃ were two to five times of those before storage, and the rate of P neu in CRCs stored at 4 ℃ was ten to twenty times of that before storage. PLA in the CRCs separated from plasma rose to a high level at the first day of preparation. The concentration of PLA in CRCs reached the highest level at one week after stored at 4 ℃ and then decreased, but a high concentration was retained all the while. P neu is the major part (about 80%～90%) of whole PLA in CRCs. PLA was not detectable by flow cytometry during the whole storage course. The results suggested that high concentrated PLA was detected in CRCs stored at 4 ℃, which could be prevented by leucocyte depleting filtration.
出处
《解放军医学杂志》
CAS
CSCD
北大核心
2002年第12期1096-1097,共2页
Medical Journal of Chinese People's Liberation Army
基金
国家自然科学基金资助课题 (编号 39970 81 2 1 )
