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小鼠S100A3蛋白的表达纯化及其多克隆抗体的制备

Expression and Purification of Mouse S100A3 and Preparation of Polyclonal Antibody
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摘要 富含半胱氨酸的S100A3是S100蛋白家族成员,其表达具有组织和细胞特异性。本研究利用基因克隆技术将小鼠S100A3克隆到原核表达载体p ET28a(+)上,经过原核表达纯化获得了高纯度的目的蛋白,制备了高效价的兔多克隆抗体。重组小鼠S100A3蛋白原核表达和纯化方法的建立及多克隆抗体的制备为其生物学功能研究提供了材料,并对其它S100蛋白家族的表达纯化具有一定的参考意义。 Cysteine-rich S100A3 is a member of S100 protein family. S100A3 shows a tissue- and cell-specific expression pattern. We constructed the mouse S100A3 expression vector, obtained S100A3 protein with high purity through expression and purification, and prepared high titer polyclonal antibody against S100A3. This strategy could facilitate the investigation of the biological function and have reference values for the expression and purification of other S100 proteins.
作者 关文 李京敬 邓晴 余晓岚 郜尽 周亮 夏鹏 韩伟 俞雁
机构地区 上海交通大学农业与生物学院 上海交通大学药学院
出处 《基因组学与应用生物学》 CAS CSCD 北大核心 2015年第8期1705-1711,共7页 Genomics and Applied Biology
基金 国家自然科学基金(No.81273573) 上海市科学基金(No.11431921300)共同资助
关键词 S100A3 蛋白表达 纯化 多克隆抗体 S100A3 Protein expression Purification Polyclonal antibody
分类号 Q78 [生物学—分子生物学]
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基因组学与应用生物学
2015年 第8期

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