摘要
本文应用沙门氏菌单克隆抗体建立了ELISA检测鼠伤寒沙门氏菌(STM)的方法,并与协同凝集方法对临床分离的162株STM进行对比鉴定研究,结果表明ELISA其方法灵敏高度,可检出5×10~2菌/ml,特异性强,且结果准确客观,对鼠伤寒沙门氏菌0-4抗原的检出率为100％,抗原H-i的检出率为96.3％,同时结果表明所用沙门氏菌McAb特异性和敏感性都达到了目前所用的沙门氏菌诊断因子血清的标准,具有推广应用的前景。
We developed an ELISA method using monoclonal antibodies against Salmonella typhimurium ( McAb, STM) for detection of STM. 162 clinical isolates of STM were investigated by ELISA and staphylococcus protein A coagglutination test ( SPA- CoA) simultaneously, and a comparison was made between these two methods. The results indicated that the ELISA method was sensitive enough to detect 5×102/ml of STM, and its specificity was also high; the detection rate of STM 0-4 antigen is 100% while that of H-i antigen is 96.3%. Furthermore, this study revealed that the sensitivity and specificity of the McAbs used were all comparable to the current standard of the diagnostic serum factor for Salmonella. The experimental method showed a promising prospect of application.
关键词
ELISA
鼠伤寒菌
单克隆抗体
Salmonella typhimurium
antibodies, monoclonal
ELISA
