摘要
采用形成愈伤组织诱导出苗的方法进行了非洲菊组织培养快速繁殖试验。试验结果 ,花蕾作外植体直径在 0 .6～ 1.5 cm均能诱导出苗 ;最适不定芽诱导培养基为 MS+ 10 mg/ L 6 - BA+ 0 .5 mg/ L IAA+3%蔗糖 + 0 .6 %琼脂 ;最适增殖培养基为 MS+ 2 mg/ L 6 - BA+ 0 .2 mg/ L NAA+ 3%蔗糖 + 0 .6 %琼脂 ,增殖倍数达 5～ 6倍 ;最适生根培养基为 MS+ 0 .0 5 mg/ L IBA + 3%蔗糖 + 0 .6 %琼脂 ,培养 15 d,生根率达 10 0 % ;移栽最适基质配方为泥炭∶珍珠岩 =1∶ 2 ,移栽最适温度为 12～ 2 8℃ ,移栽成活率达 90 %以上。
The adventitious buds of Gerbera jamesonil Bolus can occur from callus of initiate explants.The explants are buds with diameter 0.6～1.5cm. The optimum medium for inducing adventitious buds from explants is MS with 10mg/L 6 BA +0.5mg/L IAA, 3%sucrose and 0.6% agar. The optimum medium for adventitious buds multiplication is MS with 2mg/L 6 BA+0.2mg/L NAA+3%sucrose and 0.6% agar. Which makes the multiplication rate reach 5～6 times during 20 days. The optimum medium for routing is MS with 0.05mg/L IBA, 3% sucrose and 0.6% agar, which makes the rooting rate reach 100% during 15 days. The optimum medium for transplanting plants is peat(1)∶perlite(2),which makes the survival rate of plants above 90%. The optimum temperature for transplanting is 12～28℃.
出处
《贵州农业科学》
CAS
2002年第6期34-35,共2页
Guizhou Agricultural Sciences
基金
贵州省农科院重点课题"非洲菊引种栽培试验"内容之一
