摘要
目的 观察经空肠输注大黄煎液对重症急性胰腺炎大鼠肠道屏障功能的保护作用。方法 采用胰管逆行注射 3.5 %牛黄胆酸钠法建立大鼠重症急性胰腺炎 (SAP)模型 ,假手术组用 0 .9%氯化钠注射液替代牛黄胆酸钠 ,所有大鼠均经胃造瘘口空肠置管。SAP组随机分为输注大黄煎液组 (SAP A组 )及 0 .9%氯化钠注射液组 (SAP B组 ) 2个亚组 (每组 1 2只 ) ,假手术 (SO)组 8只。SAP造模成功 48h后 ,SAP组开始输注大黄煎液或 0 .9%氯化钠注射液。检测指标包括 :肠道转运系数、血清淀粉酶、脏器细菌移位率、胰腺病理评分、血浆D 乳酸等。结果 ①SAP造模成功后 72hSAP A组大鼠的肠道转运系数高于SAP B组 (P <0 .0 5 )而低于SO组 (P <0 .0 1 ) ,脏器细菌移位率、血浆D 乳酸水平低于SAP B组 (P <0 .0 5 )而高于SO组 (P <0 .0 1 ) ;②SAP A组与SAP B组在血淀粉酶和胰腺病理评分方面无显著性差异(P >0 .0 5 )。结论 SAP早期经空肠输注大黄煎液有助于肠道功能的恢复和对肠道屏障功能的保护 ,但对SAP的病程。
Objective To observe the effect of Rhubarb on the intestinal barrier of rats with severe acute pancreatitis (SAP).Methods SAP model of rats was induced by injecting adversely the 3.5% sodium taurocholate into the biliopancreatic duct, and saline was used in the rats of sham operation group instead of sodium taurocholate, and catheters were placed into the jejunum via gastrostomy for all rats. The 24 rats with SAP were randomly divided into two groups, group A(n=12) used Rhubarb decoction and group B used saline instead, and there were 8 rats in sham operation group. Rhubarb decoction was used after 48 hours of model making. The indexes observed were intestinal transmit index, bacterial translocation rate, serum amylase, histological score of the pancreas and the level of D lactate. Results Group A had a significantly higher intestinal transmit index, with a lower translocation organ bacterial rate and plasma D lactate, compared with that of group B (P<0.05). There was no difference in serum amylase and histological scores between the two groups (P>0.05).Conclusion The jejunal administration of rhubarb decoction can help to the restoration of intestinal function and the protection of intestinal barrier, but which can't improve the state of SAP.
出处
《河北中医》
2002年第11期875-878,共4页
Hebei Journal of Traditional Chinese Medicine
