摘要
目的 研究泰素帝 (TXT)在体外对肿瘤细胞的运动、黏附及侵袭能力的影响 ,探讨TXT治疗胰腺癌的作用。方法 用Transwell小室重建基底膜侵袭模型、趋化运动模型及采用细胞黏附分析方法检测TXT对胰腺癌细胞株SUIT -2及其耐药细胞株S2 /TXT的侵袭、运动及黏附能力的影响。结果 TXT可明显抑制SUIT -2细胞的侵袭及运动能力 ,但对其黏附能力无明显影响。与SUIT -2细胞相比 ,TXT对S2 /TXT细胞的侵袭、运动及黏附能力均无明显作用。结论 TXT可降低胰腺癌细胞的体外侵袭能力 ,但对胰腺癌耐药细胞株的侵袭能力无明显作用。
Objective To investigate the effects of taxotere on the mobility,adhesion and invasion ability of pancreatic tumor cells.Methods Pancreatic carcinoma cell line SUIT 2 and taxotere resistance cell line S2/TXT were exposed to 4 nmol/L(IC10 to SUIT 2) of taxotere,transwell chambers with or without reconstituted mateigel and adhesion assay were used to test the ability of cell invasion,mobility and adhesion,respectively.Results Taxotere inhibited the ability of invasion and mobility significantly,but had no effect on the adhesion ability in SUIT 2 cells.No effects on cell invasion,mobility and adhesion were found in S2/TXT cells treated with taxotere.Conclusion The reduced ability of invasion in pancreatic carcinoma in vitro treated with taxotere may be a result of inhibition effect of taxotere on cell mobility and taxotere has no effect on invasiveness in taxotere resistance pancreatic carcinoma cells.
出处
《实用癌症杂志》
2002年第6期574-576,共3页
The Practical Journal of Cancer
