摘要
利用SSR标记研究了 10个玉米自交系的遗传变异 ,初步进行了杂种优势群划分。 10对SSR引物在供试材料中共检测出 4 0个等位基因变异 ,每对引物检测出等位基因 2～ 5个 ,平均检出4个 ,平均多态信息含量值为 0 .6 5,10个自交系间平均遗传相似系数为 0 .2 7。聚类分析结果表明 ,供试自交系可归为两个类群 ,每个类群又分别由两个亚群组成。利用SSR标记可以对玉米自交系的遗传变异进行初步分析 ,并可用于杂种优势群划分。
Simple sequence repeats(SSRs)were used to detect genetic variation among 10 maize inbred lines.10 SSR primers produced 40 polymorphic amplified fragments.The average number of alleles per SSR locus was 4.0 with a rang from 2 to 5.The polymorphism information content(PIC)in average was 0.65.Average Genetic Similarity was 0.27,cluster analysis state that the 10 inbred lines of the sample were classified into 2groups and 4 sub groups.Consequently SSR makers could be used for measuring genetic variation and assigning maize inbred line to hyterotic groups.
出处
《山西农业科学》
2002年第4期19-22,共4页
Journal of Shanxi Agricultural Sciences
基金
20 0 1年度山西省回国留学人员科研资助项目 ( 2 0 0 168)部分内容
