摘要
从发病濒死的虎纹蛙(Rana tigrina rugulosa)蝌蚪中分离到一种病毒。在25℃条件下,该病毒能在鲤鱼表皮瘤细胞系(EPC),胖头肌肉细胞系(FHM)和草鱼性腺细胞系(CO)三种鱼类细胞上产生空斑状的细胞病理变化(CPE)。该病毒对氯仿,热(56℃,30min)和酸(pH3)敏感。其体外培养的适合增殖温度范围为20～30℃。 电镜下观察,病毒为对称的二十面体,切面正六边形,对角直径125nm左右。
In vitro culture and physico-chemical properties of a virus isolated from moribund Rana tigrina rugulosa were investigated. The results showed that the virus multiplied on EPC(epithelioma papillosum cyprini ), CO (ovary cells of grass carp) and FHM( muscle cells of fathead minnow) cell lines, and caused similar round lytic plaque cytopathic effect (CPE) at the temperature of 251; The optimal temperatures for the virus propagation in vitro ranged between 20 and 30℃When incubated at 56℃for 30 min, or in chloroform and pH3, the virus lost infectivity. Under transmission electron microscope, the virus particles in infected EPC exhibited icosahedral symmetry with hexagonal cross-sections and 125nm in diameter.
出处
《水产学报》
CAS
CSCD
北大核心
2002年第4期363-367,共5页
Journal of Fisheries of China
关键词
虹彩病毒
虎纹蛙
病毒
体外培养
理化特性
Rana tigrina rugulosa
iridovirus
in vitro culture
physico-chemical properties
